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BrdU Mouse mAb - Primary antibody, IgG1

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Mouse
  • Reactive species: Species independent
  • Isotype: Mouse IgG1
  • Clone Number: AB06/4C1
  • Conjugation: Unconjugated
In stock
Item Number
Ab176205
Grouped product items
SKU Size
Availability
 Price Qty
Ab176205-10μl
10μl
≥10
$66.90
Ab176205-50μl
50μl
≥10
$199.90
Ab176205-100μl
100μl
10
$299.90
Ab176205-1ml
1ml
4
$1,599.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (AB06/4C1); Mouse anti BrdU Antibody; Flow, ICC/IF, ELISA; Unconjugated

View related series
BrdU (5) Flow Cytometry (1158)

Basic Description

Product Name BrdU Mouse mAb - Primary antibody, IgG1
Synonyms Bromodeoxyuridine antibody | BrdU antibody | Anti-BrdU antibody | 5-bromo-2-deoxyuridine antibody | BrdU Monoclonal Antibody | Anti-BrdU/5-BrdU Antibody | Anti BrdU
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species Mouse
Immunogen Bromodeoxyuridine
Positive Control Flow: Jurkat cells; ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application
Flow: 0.01 μg/mL- 2.0 μg/mL
ICC/IF: 0.5 μg/mL
Mouse anti BrdU antibody, clone 4C1, is suitable for detecting incorporated BrdU in a wide variety of cell types.

Product Properties

Isotype IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

BrdU Mouse mAb (Ab176205) - Flow Cytometry
Dot plot showing BrdU-treated (A) and DMSO-treated (B) Jurkat cells stained with Ab176205. Cells were incubated with 10 µM BrdU for 1 hour prior to being harvested. And then fixed in 100% methanol for 20 minutes at -20℃. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature.


Cells were stained with 0.1 μg/mL BrdU Mouse mAb (Ab176205) for 1 hour at room temperature. Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as a secondary antibody at a 1/1000 dilution. Propidium Iodide was used to stain total DNA.

BrdU Mouse mAb (Ab176205) - Flow Cytometry
Flow cytometry analysis of BrdU (red line) in Jurkat cells. The cells were treated with 10μM BrdU for 1 hour. And then fixed in 100% methanol for 20 minutes at -20℃. An acid denaturation method was used to prepare the cells before labeling with BrdU Mouse mAb (Ab176205) to detect the incorporated BrdU. Cells were then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at room temperature in the dark. Unlabeled sample (black line) was used as a negative control.

BrdU Mouse mAb (Ab176205) - ICC/IF
IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL.

 
beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue).

 
Images were taken on the confocal laser scanning microscope.

BrdU Mouse mAb (Ab176205) - ICC/IF
IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL.

 
beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue).

 
Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

0.5 μg/mL

Flow Cytometry

0.01 μg/mL- 2.0 μg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

6 results found

Lot Number Certificate Type Date Item
ZJ24F0102485 Certificate of Analysis Jan 18, 2024 Ab176205
ZJ24F0102484 Certificate of Analysis Jan 18, 2024 Ab176205
ZJ24F0102483 Certificate of Analysis Jan 18, 2024 Ab176205
ZJ24F0102474 Certificate of Analysis Jan 18, 2024 Ab176205
ZJ23F0901024 Certificate of Analysis Sep 11, 2023 Ab176205
ZJ23F0901023 Certificate of Analysis Sep 11, 2023 Ab176205

Documents & Articles

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Experimental Guide of Sample Preparation for Flow Cytometry
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Immunofluorescence Staining Protocol (Methanol permeabilization)
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Flow Cytometry Using Methanol Permeabilization
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Sodium Azide Removal Protocol
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