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Annealing Buffer for DNA Oligos (10X) - BioReagent, PCR Reagent, Suitable for molecular biology, sterile, for DNA and RNA applications, 10X, high purity

COA COA
In stock
Item Number
A743817
Grouped product items
SKU Size
Availability
 Price Qty
A743817-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$20.90
A743817-5ml
5ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$72.90
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View related series
Annealing Buffer (2) PCR (7)

Basic Description

Specifications & Purity BioReagent, PCR Reagent, Suitable for molecular biology, sterile, for DNA and RNA applications, 10X
Stability And Storage Store at -20 ℃. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Grade BioReagent, for DNA and RNA applications, for molecular biology, PCR Reagent, sterile
Product Description

Product Introduction:

Aladdin's Annealing Buffer for DNA Oligos (10X) is a buffer solution that has been verified through multiple experiments for the annealing of DNA oligonucleotides. This annealing buffer is not only suitable for the routine annealing of DNA oligos but is especially designed for the annealing of DNA oligos used in RNAi (also known as siRNA) plasmid construction, which are often difficult to anneal. DNA oligos used for RNAi plasmid construction typically contain about 20 complementary sequences are and prone to forming hairpin structures, which can interfere with the correct annealing of the two DNA oligos. Using Aladdin's Annealing Buffer for DNA Oligos (10X) can effectively prevent the formation of hairpin structures in DNA oligos, and the annealed oligos can be directly ligated to purified plasmids that have been digested with restriction enzymes. This typically results in a high yield of positive clones after transformation. The operation with this product is very simple; just mix the DNA oligos to be annealed with the Annealing Buffer for DNA Oligos (10X) in a certain proportion and place the mixture in a PCR machine. The annealing process can be completed in about 20 minutes.

Precautions:

The Annealing Buffer for DNA Oligos (10X) is only suitable for the annealing of DNA oligos and should not be used for the annealing of RNA oligos.

This product is intended for research use by professionals only. It is not to be used for clinical diagnosis or treatment, nor is it to be used for food or pharmaceutical purposes. It should not be stored in ordinary residential areas.

For your safety and health, please wear a lab coat and disposable gloves when operating.

Names and Identifiers

PH 8

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Alternative Products

  1. Annealing Buffer for RNA Oligos (10X)
    Annealing Buffer for RNA Oligos (10X)

    Starting at $20.90

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Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.
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