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5-Carboxyfluorescein - 95%, high purity , CAS No.76823-03-5

14 Citations COA COA
    Grade & Purity:
  • ≥95%
In stock
Item Number
C105530
Grouped product items
SKU Size
Availability
 Price Qty
C105530-25mg
25mg
5
$17.90
C105530-100mg
100mg
5
$58.90
C105530-250mg
250mg
5
$88.90
C105530-500mg
500mg
5
$117.90
C105530-1g
1g
5
$212.90
C105530-5g
5g
1
$955.90
C105530-25g
25g
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$4,301.90
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Bulk Order  SDS  DATASHEET  Specification Sheet

Amine-reactive fluorescent label

View related series
Fluorescent Dye (372) Six-Membered Heterocyclic Compounds (1696) Spectroscopic reagent (233)

Basic Description

Synonyms Q27122687 | 3',6'-Dihydroxy-3-oxo-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid | BCP22613 | DTXSID30227642 | 5-FAM | 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid | AS-32304 | PD012213 | Spiro(isobenzofuran-1(3H),9'-(9H)
Specifications & Purity ≥95%
Biochemical and Physiological Mechanisms Amine-reactive fluorescent label. 6-FAM isomer. Polar tracer. Covalently labels peptides and proteins. 5-CFDA N-succinimidyl ester and 5-CFDA hydrolytic product.
Storage Temp Store at 2-8°C
Shipped In
Wet ice
This product requires cold chain shipping. Ground and other economy services are not available.
Note Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
Superclass Organoheterocyclic compounds
Class Benzopyrans
Subclass 1-benzopyrans
Intermediate Tree Nodes Dibenzopyrans
Direct Parent Xanthenes
Alternative Parents M-phthalic acid and derivatives  Diarylethers  Phthalides  Benzofuranones  Isobenzofurans  1-hydroxy-2-unsubstituted benzenoids  Dicarboxylic acids and derivatives  Lactones  Carboxylic acid esters  Oxacyclic compounds  Carboxylic acids  Organic oxides  Hydrocarbon derivatives  
Molecular Framework Aromatic heteropolycyclic compounds
Substituents Xanthene - Meta_phthalic_acid - Diaryl ether - Benzofuranone - Phthalide - Isobenzofuranone - Isobenzofuran - Isocoumaran - 1-hydroxy-2-unsubstituted benzenoid - Benzenoid - Dicarboxylic acid or derivatives - Lactone - Carboxylic acid ester - Oxacycle - Ether - Carboxylic acid - Carboxylic acid derivative - Hydrocarbon derivative - Organic oxide - Organooxygen compound - Organic oxygen compound - Aromatic heteropolycyclic compound
Description This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
External Descriptors monocarboxylic acid

Names and Identifiers

Pubchem Sid 488187777
Pubchem Sid Url https://pubchem.ncbi.nlm.nih.gov/substance/488187777
IUPAC Name 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
INCHI InChI=1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
InChIKey NJYVEMPWNAYQQN-UHFFFAOYSA-N
Smiles C1=CC2=C(C=C1C(=O)O)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
Isomeric SMILES C1=CC2=C(C=C1C(=O)O)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
WGK Germany 3
Molecular Weight 376.32
Beilstein 57037
Reaxy-Rn 57037
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=57037&ln=

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

25 results found

Lot Number Certificate Type Date Item
K2011125 Certificate of Analysis Aug 16, 2024 C105530
D2403383 Certificate of Analysis Mar 08, 2024 C105530
F1928038 Certificate of Analysis Apr 13, 2023 C105530
C2329688 Certificate of Analysis Feb 21, 2023 C105530
C2329692 Certificate of Analysis Feb 21, 2023 C105530
C2329674 Certificate of Analysis Feb 21, 2023 C105530
C2329676 Certificate of Analysis Feb 21, 2023 C105530
C2329677 Certificate of Analysis Feb 21, 2023 C105530
C2329691 Certificate of Analysis Feb 21, 2023 C105530
C2329678 Certificate of Analysis Feb 21, 2023 C105530
C2329689 Certificate of Analysis Feb 21, 2023 C105530
C2329679 Certificate of Analysis Feb 21, 2023 C105530
C2329680 Certificate of Analysis Feb 21, 2023 C105530
C2329690 Certificate of Analysis Feb 21, 2023 C105530
K1424008 Certificate of Analysis Aug 09, 2022 C105530
I1814032 Certificate of Analysis Jul 18, 2022 C105530
B2211302 Certificate of Analysis Dec 20, 2021 C105530
B2211376 Certificate of Analysis Dec 20, 2021 C105530
B2211219 Certificate of Analysis Dec 20, 2021 C105530
B2211301 Certificate of Analysis Dec 20, 2021 C105530
B2211299 Certificate of Analysis Dec 20, 2021 C105530
B2211297 Certificate of Analysis Dec 20, 2021 C105530
J2307063 Certificate of Analysis Dec 20, 2021 C105530
J2424309 Certificate of Analysis Dec 20, 2021 C105530
H2419103 Certificate of Analysis Dec 20, 2021 C105530

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Chemical and Physical Properties

Solubility soluble in DMF and H2O and DMSO
Sensitivity light & Moisture sensitive
Molecular Weight 376.300 g/mol
XLogP3 2.900
Hydrogen Bond Donor Count 3
Hydrogen Bond Acceptor Count 7
Rotatable Bond Count 1
Exact Mass 376.058 Da
Monoisotopic Mass 376.058 Da
Topological Polar Surface Area 113.000 Ų
Heavy Atom Count 28
Formal Charge 0
Complexity 637.000
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
The total count of all stereochemical bonds 0
Covalently-Bonded Unit Count 1

Citations of This Product

How to Cite Aladdin Scientific Products?
1. Xiaowei Cao, Zhengqing Liu, Xiaogang Qin, Yuexing Gu, Yong Huang, Yayun Qian, Zhenguang Wang, Hongbo Li, Qunshan Zhu, Wei Wei.  (2024)  LoC-SERS platform for rapid and sensitive detection of colorectal cancer protein biomarkers.  TALANTA,  270  (125563). 
2. Min Li, Honglang Lu, Menghan Pi, Hui Zhou, Yufei Wang, Bin Yan, Wei Cui, Rong Ran.  (2023)  Water-Induced Phase Separation for Anti-Swelling Hydrogel Adhesives in Underwater Soft Electronics.  Advanced Science,    (2304780). 
3. Wang Youwei, Chen Bing, Fan Jiang, Wang Zhong.  (2023)  A simple and efficient strategy for trace detection of ferroptosis-related miRNAs based on novel hydrophobic paper-based plasmonic substrate and “inverse molecular sentinel (iMS)” nanoprobes.  Frontiers in Bioengineering and Biotechnology,  11   
4. Weizhou Sha, Mingzhu Wang, Wei Wang, Zhi Yuan.  (2022)  pH-Responsive nanoprobes for tumour fluorescence imaging based on spirolactam rhodamine.  Materials Chemistry Frontiers,  6  (19): (2869-2879). 
5. Xiao-Qin Yang, Li-Wen Bai, Yu Chen, Yue-Xiao Lin, Hua Xiang, Ting-Ting Xiang, Shuang-Xing Zhu, Li Zhou, Kai Li, Xinxiang Lei.  (2022)  Peptide probes with high affinity to target protein selection by phage display and characterization using biophysical approaches.  NEW JOURNAL OF CHEMISTRY,  46  (21): (10299-10307). 
6. Zechun Dang, Jizheng Sun, Jiaqi Fan, Jinqi Li, Xinlei Li, Tongsheng Chen.  (2021)  Zinc oxide spiky nanoparticles: A promising nanomaterial for killing tumor cells.  Materials Science & Engineering C-Materials for Biological Applications,  124  (112071). 
7. Di Wang, Erjun Sun.  (2020)  Loading carboxyfluorescein into porous SiO2 spheres and UCNPs-cored porous SiO2 spheres: Emission turn-on sensing with a warning signal for tumor-biomarker 5-HIAA urine test.  MICROPOROUS AND MESOPOROUS MATERIALS,  299  (110131). 
8. Xiuxia Sun, Linyan Yao, Caihong Fu, Liting Luo, Jie Wang, Jianxi Xiao.  (2019)  Detection of target collagen peptides with single amino acid mutation using two fluorescent peptide probes.  Journal of Materials Chemistry B,  7  (48): (7676-7682). 
9. Kunyuan Luo, Yuhong Yang, Zhengzhong Shao.  (2015)  Physically Crosslinked Biocompatible Silk-Fibroin-Based Hydrogels with High Mechanical Performance.  ADVANCED FUNCTIONAL MATERIALS,  26  (6): (872-880). 
10. Qianqian Long, Xinmin Zhao, Lili Gao, Mengyuan Liu, Feng Pan, Xihui Gao, Changyou Zhan, Yang Chen, Jialei Wang, Jun Qian.  (2024)  Effects of Surface IR783 Density on the In Vivo Behavior and Imaging Performance of Liposomes.  Pharmaceutics,  16  (6): (744). 
11. Ying Luo, Da Lei, Maohua Li, Yuansheng Ge, Jiguang Li, Baiyi Zu, Jun Yao, Xincun Dou.  (2024)  Fluorophore branching boosted photo-induced energy transfer in UiO-66 for ultrasensitive and instant hydrazine sensing.  Journal of Materials Chemistry A,     
12. Xueqin Zhu, Xiaoxi Wang, Zimai Liu, Bing Jiang, Zonghong He, Sijia Liu, Yahong Wu, Zixian Wu, Tiantian Zhang, Meiyi Liu, Kai Li, Xiaoshuang Niu, Yanfeng Gao.  (2024)  Peroxidase-Like Nanozyme Activates the cGAS-STING Pathway via ROS-Induced mtDNA Release for Cancer Immunotherapy.  ADVANCED FUNCTIONAL MATERIALS,    (2401576). 
13. Ning Xia, Fengli Gao, Gang Liu, Yong Chang, Lin Liu.  (2025)  Pyrroloquinoline quinone exhibiting peroxidase-mimicking property for colorimetric assays.  ANALYTICA CHIMICA ACTA,  1335  (343468). 
14. Jiliang Zhang, Shuya Zhang, Cairong Yan, Jiaqi Bi, Xia Han, Honglai Liu.  (2024)  Tint-Adjustable Pickering Emulsion Sunscreen Based on Polydopamine-Coated Silica Nanoparticles.  ACS Applied Nano Materials,  7  (13): (15365-15375). 

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