Z-WEHD-FMK is a potent, cell-permeable and irreversible caspase-1/5 inhibitor. Z-WEHD-FMK also exhibits a robust inhibitory effect on cathepsin B activity ( IC 50 =6 μM). Z-WEHD-FMK can be used to investigate cells for evidence of apoptosis.
Storage Temp
Desiccated,Store at -80°C
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Product Description
Z-WEHD-FMK is a potent, cell-permeable and irreversible caspase-1/5 inhibitor. Z-WEHD-FMK also exhibits a robust inhibitory effect on cathepsin B activity ( IC 50 =6 μM). Z-WEHD-FMK can be used to investigate cells for evidence of apoptosis.
In Vitro
Z-WEHD-FMK (80 μM; 9 hours) elicits a near-complete blockage of C. trachomatis -induced cleavage of golgin-84 and increases GM130 expression in cells. Z-WEHD-FMK (30 min before being exposed to E. piscicida ) effectively inhibits 0909I E. piscicida induced ZF4 cells cytotoxicity and pyroptotic morphology. And in addition, it also inhibits the cytotoxicity induced by cytosolic LPS delivery. Z-WEHD-FMK (20 μM;18-24 hours following Cr 3+ ,Ni 2+ , and Co 2+ ) significantly induces a decrease of 76% to 86% in IL-1β release with 200 to 400 ppm Cr 3+ , it also induces a decrease of 35% to 45% with 48 ppm Ni 2+ or higher, Finally, this caspase-1 inhibitor induced a decrease with 6 ppm Co 2+ , down to a level below the detection threshold, and a decrease of 40% to 48% with 12 to 24 ppm Co 2+ in bone marrow-derived macrophages (BMDM). MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: C. trachomatis - or mock-infected HeLa cells Concentration: 80 μM Incubation Time: 9 hours Result: Increased golgin-84 and GM130 expression. Cell Viability AssayCell Line: Mycoplasma free-ZF4 cells Concentration: Incubation Time: 30 min before being exposed to E. piscicida Result: Inhibited ZF4 cells cytotoxicity and pyroptotic morphology.
This compound belongs to the class of organic compounds known as hybrid peptides. These are compounds containing at least two different types of amino acids (alpha, beta, gamma, delta) linked to each other through a peptide bond.
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
