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DNA Loading Buffer (5X, GelRed) - 5X, high purity

    Grade & Purity:
  • 5X
In stock
Item Number
R751628
Grouped product items
SKU Size
Availability
Price Qty
R751628-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$26.90
R751628-10×1ml
10×1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$78.90

Basic Description

Synonyms Red Fluorescent DNA Loading Buffer (5X) | 5xDNA Loading Buffer with GelRed
Specifications & Purity 5X
Stability And Storage Store at 4 ℃ for 36 months. Store at -20 ℃ for 60 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Product Description

Product Introduction:
GelRed is a novel nucleic acid stain. This unique oil-soluble macromolecule is non-volatile, non-inhalable, and cannot penetrate cell membranes to enter living cells. Additionally, it exhibits no mutagenicity at the gel-staining concentration. GelRed is characterized by its safety in use and high sensitivity in detection. It can be used as a staining agent for various nucleic acid electrophoresis applications and is suitable for staining nucleic acids of all fragment sizes. GelRed is an ideal alternative to ethidium bromide (EB). DNA Loading Buffer (5X, GelRed) needs to be mixed with the sample for staining.
Composition: 10 mM Tris-HCl, 5 mM EDTA, pH 7.6, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, GelRed Nucleic Acid Stain.
Usage Instructions:
1. Use in combination with Aladdin DNA Marker.
2. Mix 4 μl of sample (<500 ng) with 1 μl of DNA Loading Buffer (5X, GelRed). Allow the mixture to stand at room temperature for 2 minutes before loading.
3. It is recommended to use 1.0-2.0% agarose gel, with a voltage of 4-10 V/cm. Perform electrophoresis using 1x TAE (preferred) or 0.5x TBE buffer. Note: Replace the electrophoresis buffer promptly and use freshly prepared gels to achieve optimal results.
4. No additional stains are required in the agarose gel. After electrophoresis, the bands can be directly visualized under UV light. The stain is compatible with commonly used UV transilluminators.


Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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