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Tyrosinase from mushroom, CAS No.9002-10-2

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Item Number
T128536
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T128536-25KU
25KU
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$194.90
T128536-100KU
100KU
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$539.90

Basic Description

Product Name Tyrosinase from mushroom, CAS No.9002-10-2
Synonyms Polyphenol Oxidase | Catechol Oxidase | Monophenol Monooxygenase | Monophenol | dihydroxyphenylalanine:oxygen oxidoreductase
Grade EnzymoPure™
Product Description

Inhibitors
Compounds that complex with copper. The enzyme is also inhibited competitively by benzoic acid with respect to catechol and by cyanide with respect to oxygen (Duckworth and Coleman 1970).
Stability
The lyophilized preparation is stable for 6-12 months when stored at -20°C.
Activity
Polyphenol oxidase is an oxygen transferring enzyme. Besides using O2 to catalyze the dehydrogenation of catechols to orthoquinones and the orthohydroxylation of phenols to catechols, a peroxidase activity has been reported on by Strothkamp and Mason (1974). Kinetic studies have been reported by Kertész et al. (1971). See also the review by Malmström and Rydén.
Polyphenol Oxidase Assay
Method
Polyphenol oxidase oxidizes tyrosine to dihydroxyphenylalanine which in turn is oxidized to o-quinone. The latter is accompanied by an increase in absorbance at 280 nm. The rate of increase is proportional to enzyme concentration and linear during a period of 5-10 minutes after an initial lag. One unit causes a change in absorbance at 280 nm of 0.001 per minute at 25°C, pH 6.5 under the specified conditions.
Reagents
0.5 M Potassium phosphate buffer, pH 6.5
0.001 M L-Tyrosine
Enzyme
Dissolve at a concentration of 1 mg/ml in reagent grade water. Dilute further in reagent grade water to a concentration of 200-400 units/ml.
Procedure
Adjust the spectrophotometer to 280 nm and 25°C. Pipette into each cuvette as follows:
0.5 M Phosphate buffer, pH 6.5 1.0 ml
0.001 M L-Tyrosine 1.0 ml
Reagent grade water 0.9 ml
Oxygenate this reaction mixture by bubbling oxygen into cuvettes through a capillary tube for 4-5 minutes. Transfer cuvettes to the spectrophotometer and record A280 for 4-5 minutes to achieve temperature equilibration and to establish blank rate, if any. Add 0.1 ml of appropriately diluted enzyme and record A280 for 10-12 minutes. Determine ΔA280 from the linear portion of the curve. A non-linear "lag" of 2-3 minutes can be expected.

Calculation


Specifications & Purity EnzymoPure™, ≥500 units/mg dry weight
Biochemical and Physiological Mechanisms Converts tyrosine to L-DOPA or tyramine to dopamine.

Storage and Shipping

Concentration ≥500 units/mg dry weight
Storage Temp Store at -20°C
Shipped In Ice chest + Ice pads
CAS 9002-10-2
Enzyme Commission Number I.U.B.: 1.14.18.1
Unit definition Unit Definition: One unit causes an increase in the absorbance at 280 nm of 0.001 per minute at 25°C, pH 6.5, using L-tyrosine as substrate.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

19 results found

Lot Number Certificate Type Date Item
E2506337 Certificate of Analysis Feb 27, 2025 T128536
E2506338 Certificate of Analysis Feb 27, 2025 T128536
L2418484 Certificate of Analysis Nov 28, 2024 T128536
L2418485 Certificate of Analysis Nov 28, 2024 T128536
K2401643 Certificate of Analysis Oct 22, 2024 T128536
K2401642 Certificate of Analysis Oct 22, 2024 T128536
G2416542 Certificate of Analysis Jul 09, 2024 T128536
G2416540 Certificate of Analysis Jul 09, 2024 T128536
G2416541 Certificate of Analysis Jul 09, 2024 T128536
E2428377 Certificate of Analysis May 22, 2024 T128536
E2428378 Certificate of Analysis May 22, 2024 T128536
L2304373 Certificate of Analysis Nov 21, 2023 T128536
L2304376 Certificate of Analysis Nov 21, 2023 T128536
H2318746 Certificate of Analysis Aug 15, 2023 T128536
H2318747 Certificate of Analysis Aug 15, 2023 T128536
J2225671 Certificate of Analysis Oct 14, 2022 T128536
J2225698 Certificate of Analysis Oct 14, 2022 T128536
G2416043 Certificate of Analysis Dec 14, 2021 T128536
A2411063 Certificate of Analysis Apr 30, 2021 T128536

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Citations of This Product

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