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Trypsin solution from porcine pancreas

Grade & Purity:

1 ×, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red

Accession #: P00761

In stock

Item Number

T755941

Grouped product items
SKU	Size	Availability	Price	Qty
T755941-100ml	100ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$16.90
T755941-500ml	500ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$46.90

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Click to view protein information: PRSS1

Basic Description

Product Name	Trypsin solution from porcine pancreas
Grade	BioReagent, for cell culture, sterile-filtered
Product Description	Trypsin solution from porcine pancreas has been used for keratinocyte isolation and staining. It has also been used as a component of CMF-Steinberg′s solution to replace heart tissues after centrifugation in a study to culture embryonic cardiomyocytes from Mexican axolotl. For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.
Specifications & Purity	sterile-filtered, BioReagent, for cell culture, 1 ×, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
Biochemical and Physiological Mechanisms	Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxy
Accession #	P00761

Storage and Shipping

Concentration	1 ×, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
Storage Temp	Store at -20°C
Shipped In	Ice chest + Ice pads

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