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Trypan Blue - 10mM in DMSO, high purity , CAS No.72-57-1

38 Citations

COA

Grade & Purity:

10mM in DMSO

Cas Number: 72-57-1
Molecular Weight: 960.81
Beilstein Registry Number: 4360496
EC Number: 200-786-7
PubChem CID: 6296

In stock

Item Number

T425683

Grouped product items
SKU	Size	Availability	Price	Qty
T425683-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90

View related series

Cell Membrane Permeability Detection (6) Compound libraries (12325) Live/Dead Cell Staining (9)

Basic Description

Synonyms	TRYPAN BLUE \| Direct Blue 14 \| 72-57-1 \| Niagara Blue \| C.I. Direct Blue 14 \| VisionBlue \| Benzamine Blue \| Dianil blue \| Diamine Blue \| Benzo Blue \| Congo Blue \| Naphthylamine Blue \| Diphenyl Blue \| C.I. 23850 \| Niagara Blue 3B \| trypan-blue \| Diamineblue \| Dianilblau \| Pyrotropblau \| Parkib
Specifications & Purity	10mM in DMSO
Biochemical and Physiological Mechanisms	The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. Whil
Storage Temp	Protected from light,Store at -80°C
Shipped In	Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available.
Product Description	Product Description The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol. Product Application Trypan blue has been used in cell viability assay, to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution, to stain plant tissue in order to to visualize cell death.

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Benzenoids
  - Class Benzene and substituted derivatives
    - Subclass Biphenyls and derivatives
      - Level5 Benzidines
        Level6 3,3'-disubstituted benzidines

Kingdom	Organic compounds
Superclass	Benzenoids
Class	Benzene and substituted derivatives
Subclass	Biphenyls and derivatives
Intermediate Tree Nodes	Benzidines
Direct Parent	3,3'-disubstituted benzidines
Alternative Parents	2-naphthalene sulfonic acids and derivatives 2-naphthalene sulfonates 1-sulfo,2-unsubstituted aromatic compounds Toluenes Phenoxides Sulfonyls Organosulfonic acids Azo compounds Propargyl-type 1,3-dipolar organic compounds Primary amines Organooxygen compounds Organic sodium salts Organic oxides Hydrocarbon derivatives Organic cations
Molecular Framework	Aromatic homopolycyclic compounds
Substituents	3,3'-disubstituted benzidine - 2-naphthalene sulfonate - Naphthalene sulfonic acid or derivatives - 2-naphthalene sulfonic acid or derivatives - Naphthalene sulfonate - Naphthalene - Arylsulfonic acid or derivatives - 1-sulfo,2-unsubstituted aromatic compound - Phenoxide - Toluene - Organic sulfonic acid or derivatives - Organosulfonic acid or derivatives - Organosulfonic acid - Sulfonyl - Azo compound - Organic alkali metal salt - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Organic salt - Organic sodium salt - Amine - Primary amine - Hydrocarbon derivative - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Organic oxide - Organic oxygen compound - Organic nitrogen compound - Organic cation - Aromatic homopolycyclic compound
Description	This compound belongs to the class of organic compounds known as 3,3'-disubstituted benzidines. These are organic compounds containing a benzidine skeleton, which is substituted only at the 3- and 3'-positions.
External Descriptors	Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

Names and Identifiers

IUPAC Name	tetrasodium;5-amino-3-[[4-[4-[(8-amino-1-hydroxy-3,6-disulfonatonaphthalen-2-yl)diazenyl]-3-methylphenyl]-2-methylphenyl]diazenyl]-4-hydroxynaphthalene-2,7-disulfonate
INCHI	InChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChIKey	GLNADSQYFUSGOU-UHFFFAOYSA-J
Smiles	CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O.[Na+].[Na+].[Na+].[Na+]
Isomeric SMILES	CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C(C=C4C=C3S(=O)(=O)[O-])S(=O)(=O)[O-])N)O)C)N=NC5=C(C6=C(C=C(C=C6C=C5S(=O)(=O)[O-])S(=O)(=O)[O-])N)O.[Na+].[Na+].[Na+].[Na+]
WGK Germany	3
RTECS	QJ6475000
PubChem CID	6296
Molecular Weight	960.81
Beilstein	4360496

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Chemical and Physical Properties

Sensitivity	light sensitive
Melt Point(°C)	>300°C
Molecular Weight	960.800 g/mol
XLogP3
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	20
Rotatable Bond Count	5
Exact Mass	959.982 Da
Monoisotopic Mass	959.982 Da
Topological Polar Surface Area	404.000 Å²
Heavy Atom Count	62
Formal Charge	0
Complexity	1790.000
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
The total count of all stereochemical bonds	0
Covalently-Bonded Unit Count	5

Documents & Articles

In Vitro Cytotoxicity Testing Guide

Categories: Technical articles

Tags:

Biological buffers Nucleosides & nucleotides Stains & Dyes MTT assay method CCK-8 experiment live-dead cell staining

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Kankan Chen, Jiawei Xu, Jingjing Song, Zhonggang Liu, Zheng Guo. (2023) A facile ratiometric electrochemical sensing platform with in-situ electropolymerized thionine on effective sensing of 5-hydroxytryptamine. ELECTROANALYSIS, 36 (3): (e202300324).
2. Guangyong Zeng, Hu Zheng, Kun Zhou, Hao Shi, Size Zheng, Hui Ma, Peng Wang, Shengyan Pu. (2023) Preparation, Characterization and Application of Novel Photocatalytic Two-Dimensional Material Membrane: A Reform of Comprehensive Experimental Teaching. Sustainability, 15 (14): (11342).
3. He Zhang, Wenmei Luo, Yuping Du, Hao Shi, Guangyong Zeng, Xinxing Yan, Xiaoke Li. (2023) The g-C3N4 decorated carbon aerogel with integrated solar steam generation and photocatalysis for effective desalination and water purification. DESALINATION, 564 (116821).
4. Ling Jiang, Huan Huang, Xingyu Shi, Jian Wu, Juexian Ye, Qian Xu, Shaobin Fang, Chuanbin Wu, Rui Luo, Chao Lu, Daojun Liu. (2023) Biocontrol Microneedle Patch: A Promising Agent for Protecting Citrus Fruits from Postharvest Infection. Pharmaceutics, 15 (4): (1219).
5. Yujiao Tu, Ze Liu, Lei Jiang, Yingying Xiang, Fei Song, Lifen Meng, Xiuling Ji, Lin Yuan. (2023) Green ratiometric fluorescent dual-mode nanosensor for highly selective and sensitive detection of new coccine in food. DYES AND PIGMENTS, 210 (111024).
6. Wang Yongtao, Wang Nana, Yang Yingjun, Chen Yazhou, Zhang Zhengguo. (2022) Cellular nanomechanics derived from pattern-dependent focal adhesion and cytoskeleton to balance gene transfection of malignant osteosarcoma. JOURNAL OF NANOBIOTECHNOLOGY, 20 (1): (1-13).
7. Lin Yuan, Lei Jiang, Ze Liu, Yingying Xiang, Fei Song, Yujiao Tu. (2023) Selective and sensitive simultaneous detection of tartrazine and Hg2+ based on N-doped yellow-green fluorescent carbon dots. DYES AND PIGMENTS, 209 (110893).
8. Jin Wen, Xiaoke Li, He Zhang, Size Zheng, Caini Yi, Liu Yang, Jinwen Shi. (2023) Architecting Janus hydrogel evaporator with polydopamine-TiO2 photocatalyst for high-efficient solar desalination and purification. SEPARATION AND PURIFICATION TECHNOLOGY, 304 (122403).
9. Yingying Xiang, Yujiao Tu, Lei Jiang, Lin Yuan, Ze Liu, Qiang Xie, Xuequan Xiong, Fei Song. (2022) Orange fluorescent dual-mode nanoprobe for selective and sensitive detection of amaranth based on S,N-Doped carbon dots. DYES AND PIGMENTS, 205 (110533).
10. Siyuan Huang, Qi Su, Xiaoqiang Hou, Kuankuan Han, Shufang Ma, Bingshe Xu, Yingjun Yang. (2022) Influence of Colonies’ Morphological Cues on Cellular Uptake Capacity of Nanoparticles. Frontiers in Bioengineering and Biotechnology, 10 (922159).
11. Xiaojie Cheng, Jiahui Liao, Yue Xue, Qingquan Lin, Zhaomei Yang, Guilong Yan, Guangyong Zeng, Arijit Sengupta. (2022) Ultrahigh-flux and self-cleaning composite membrane based on BiOCl-PPy modified MXene nanosheets for contaminants removal from wastewater. JOURNAL OF MEMBRANE SCIENCE, 644 (120188).
12. Song Zhu, Fei Luo, Bin Zhu, Fei Ling, Er-Long Wang, Tian-Qiang Liu, Gao-Xue Wang. (2021) A Nanobody-Mediated Virus-Targeting Drug Delivery Platform for the Central Nervous System Viral Disease Therapy. Microbiology Spectrum, 9 (3):
13. Shanshan Wang, Hufei Wang, Yanqiu Chen, Jilai Liu, Xiaozhen He, Da Huang, Yuanzi Wu, Yonghai Chen, Zuquan Weng. (2021) Protective effects of (-)-epigallocatechin gallate and curcumin against acrylamide toxicity. TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY, 103 (2): (199-218).
14. Sihao Pan, Jiaoyu Ren, Enhui Ma, Ke Wang, Sicheng Yang, Hong Wang. (2021) Dual-Propelled Sporopollenin-Exine-Capsule Micromotors for Near-Infrared Light Triggered Degradation of Organic Pollutants. ChemNanoMat, 7 (4): (483-487).
15. Jing Zhou, Dan Yang, Guohui Liu, Siying Li, Wennan Feng, Guocheng Yang, Jin He, Yuping Shan. (2021) Highly sensitive detection of DNA damage in living cells by SERS and electrochemical measurements using a flexible gold nanoelectrode. ANALYST, 146 (7): (2321-2329).
16. Beibei Yang, Yating Dong, Yifeng Shen, Ailin Hou, Guilan Quan, Xin Pan, Chuanbin Wu. (2021) Bilayer dissolving microneedle array containing 5-fluorouracil and triamcinolone with biphasic release profile for hypertrophic scar therapy. Bioactive Materials, 6 (2400).
17. Chi Zhang, Fan Gao, Wei Wu, Wen-Xiu Qiu, Lu Zhang, Runqing Li, Ze-Nan Zhuang, Wuyang Yu, Han Cheng, Xian-Zheng Zhang. (2019) Enzyme-Driven Membrane-Targeted Chimeric Peptide for Enhanced Tumor Photodynamic Immunotherapy. ACS Nano, 13 (10): (11249–11262).
18. Hong Cheng, Ping Yuan, Guiling Fan, Linping Zhao, Rongrong Zheng, Bin Yang, Xiaozhong Qiu, Xiyong Yu, Shiying Li, Xianzheng Zhang. (2019) Chimeric peptide nanorods for plasma membrane and nuclear targeted photosensitizer delivery and enhanced photodynamic therapy. Applied Materials Today, 16 (120).
19. Maria Wasim, Aneela Sabir, Muhammad Shafiq, Rafi Ullah Khan. (2019) Fractionation of direct dyes using modified vapor grown carbon nanofibers and zirconia in cellulose acetate blend membranes. SCIENCE OF THE TOTAL ENVIRONMENT, 677 (194).
20. Xi Chen, Yi He, Yi Fan, Guangyong Zeng, Liyun Zhang. (2019) Nature-inspired polyphenol chemistry to fabricate halloysite nanotubes decorated PVDF membrane for the removal of wastewater. SEPARATION AND PURIFICATION TECHNOLOGY, 212 (326).
21. Zu-Sheng Huang, Yun-Yun Quan, Jia-Jun Mao, Yi-Li Wang, Yuekun Lai, Jing Zheng, Zhong Chen, Kun Wei, Huaqiong Li. (2019) Multifunctional superhydrophobic composite materials with remarkable mechanochemical robustness, stain repellency, oil-water separation and sound-absorption properties. CHEMICAL ENGINEERING JOURNAL, 358 (1610).
22. Yingqing Zhan, Xinyi Wan, Shuangjiang He, Qiangbin Yang, Yi He. (2018) Design of durable and efficient poly(arylene ether nitrile)/bioinspired polydopamine coated graphene oxide nanofibrous composite membrane for anionic dyes separation. CHEMICAL ENGINEERING JOURNAL, 333 (132).
23. Shi-Ying Li, Wen-Xiu Qiu, Hong Cheng, Fan Gao, Feng-Yi Cao, Xian-Zheng Zhang. (2017) A Versatile Plasma Membrane Engineered Cell Vehicle for Contact-Cell-Enhanced Photodynamic Therapy. ADVANCED FUNCTIONAL MATERIALS, 27 (12): (1604916).
24. Shi-Ying Li, Hong Cheng, Wen-Xiu Qiu, Li-Han Liu, Si Chen, Ying Hu, Bo-Ru Xie, Bin Li, Xian-Zheng Zhang. (2015) Protease-Activable Cell-Penetrating Peptide–Protoporphyrin Conjugate for Targeted Photodynamic Therapy in Vivo. ACS Applied Materials & Interfaces, 7 (51): (28319–28329).
25. Shenguang Ge, Lina Zhang, Yan Zhang, Haiyun Liu, Jiadong Huang, Mei Yan, Jinghua Yu. (2015) Electrochemical K-562 cells sensor based on origami paper device for point-of-care testing. TALANTA, 145 (12).
26. Lu Xu, Xu Xu, Hao Chen, Xingyi Li. (2013) Ocular biocompatibility and tolerance study of biodegradable polymeric micelles in the rabbit eye. COLLOIDS AND SURFACES B-BIOINTERFACES, 112 (30).
27. Shenguang Ge, Weiyan Liu, Lei Ge, Mei Yan, Jixian Yan, Jiadong Huang, Jinghua Yu. (2013) In situ assembly of porous Au-paper electrode and functionalization of magnetic silica nanoparticles with HRP via click chemistry for Microcystin-LR immunoassay. BIOSENSORS & BIOELECTRONICS, 49 (111).
28. Liu Xian-Wei, Sun Xue-Fei, Chen Jie-Jie, Huang Yu-Xi, Xie Jia-Fang, Li Wen-Wei, Sheng Guo-Ping, Zhang Yuan-Yuan, Zhao Feng, Lu Rui, Yu Han-Qing. (2013) Phenothiazine Derivative-Accelerated Microbial Extracellular Electron Transfer in Bioelectrochemical System. Scientific Reports, 3 (1): (1-7).
29. Hong Cheng,Gui-Ling Fan,Jing-Hao Fan,Ping Yuan,Fu-An Deng,Xiao-Zhong Qiu,Xi-Yong Yu,Shi-Ying Li. (2019-09-22) Epigenetics-inspired photosensitizer modification for plasma membrane-targeted photodynamic tumor therapy.. Biomaterials, 224 (119497-119497).
30. Shen Mingkui, Hou Yan, Xu Shihui, Tan Jun, Zhou Honggang, Miao Qi, Zhang Wanheng, Chen Yazhou, Wang Nana, Wang Yongtao. (2025) Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction. JOURNAL OF NANOBIOTECHNOLOGY, 23 (1): (1-18).
31. Jingsong Lu, Yupei Wang, Xiaohan Gao, Ying Li, Neema Ufurahi Pambe, Abdul Fahad, Zeping Jin, zhijun He, Zhenhu Guo, Wensheng Xie, Shi Wang, Xiaodan Sun, Xiumei Wang, Jing Yu, Shenglei Che, Guifeng Zhang, Yen Wei, Lingyun Zhao. (2024) Cancer Nutritional-Immunotherapy with NIR-II Laser Controlled ATP Release Based on Material Repurposing Strategy. Journal of Materials Chemistry B,
32. Jiali Li, Weichen Xiong, Jianxi Yang, Weifei Liao, Yihan Gao, Jinwei Chai, Jiena Wu, Shuwen Liu, Xueqing Xu. (2025) Characterization of the first antimicrobial peptide from Sea Seal with potent therapeutic effect in septic mice. BIOCHEMICAL PHARMACOLOGY, 236 (116891).
33. Xinyi Zhang, Yuelian Zhang, Huishan Zheng, Xue Yang, Shiqi Zou, Jianmin Chen. (2025) Design, fabrication, and evaluation of antimicrobial sponge microneedles for the transdermal delivery of insulin. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 206 (114586).
34. Xiang Zhong, Yuxiang Zhu, Yan Wang, Zhengtao Jia, Meng Jiang, Qiufan Sun, Jianfeng Yao. (2024) Intramolecular Quaternary Carbon Nitride Homojunction for Enhanced Visible Light Hydrogen Production. Small, (2402219).
35. Ziming Zhao, Wenhao Li, Yong Wang, Jiayu An, Qiuchen He, Su Zhan, Feng Zhou. (2025) Introducing microcrystalline cellulose to reinforce the stability of anatase TiO2 for photocatalytic hydrogen production from simulated seawater. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 109 (1055).
36. Mingwei Peng, Ziwen Heng, Dewei Ma, Bo Hou, Keke Yang, Qinglong Liu, Zhongwei Gu, Wei Liu, Siyuan Chen. (2024) Iontophoresis-Integrated Smart Microneedle Delivery Platform for Efficient Transdermal Delivery and On-Demand Insulin Release. ACS Applied Materials & Interfaces, 16 (51): (70378-70391).
37. Lan Ma, Junjie Liao, Hao Yu, Sheng Shi, Liyun Zhang, Xinchuan Ma, Xin Wen, Xinmei Shi. (2025) Rigid cross-linking: A structural stabilization strategy for enhancing the swelling and compression resistance of GO-based membranes. DESALINATION, 603 (118671).
38. Zhicheng Le, Yufeng Shou, Renee R. Li, Ling Liu, Runcheng Tan, Christopher John Charles, Zhijia Liu, Yongming Chen, Andy Tay. (2024) Sponge-Like Microneedles Spatially Sequester Chemokines and Deplete Monocytes to Alleviate Inflammatory Skin Disorders. ADVANCED FUNCTIONAL MATERIALS, (2402539).

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