Basic Description

Synonyms	3,3′,5,5′-Tetramethylbenzidine Liquid Substrate System TMB
Storage Temp	Store at 2-8°C,Argon charged
Shipped In	Wet ice This product requires cold chain shipping. Ground and other economy services are not available.
Product Description	TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

16 results found

Lot Number	Certificate Type	Date	Item
D2525347	Certificate of Analysis	Mar 24, 2025	T117927
D2525348	Certificate of Analysis	Mar 24, 2025	T117927
D2525349	Certificate of Analysis	Mar 24, 2025	T117927
K2429331	Certificate of Analysis	Mar 04, 2025	T117927
K2429330	Certificate of Analysis	Mar 04, 2025	T117927
K2429332	Certificate of Analysis	Nov 21, 2024	T117927
K2418004	Certificate of Analysis	Sep 19, 2024	T117927
F2413076	Certificate of Analysis	Jun 20, 2024	T117927
L2304055	Certificate of Analysis	Mar 21, 2024	T117927
A2429415	Certificate of Analysis	Jan 31, 2024	T117927
B2318865	Certificate of Analysis	Sep 14, 2023	T117927
B2318874	Certificate of Analysis	Sep 14, 2023	T117927
F2221525	Certificate of Analysis	Jul 12, 2023	T117927
B2318877	Certificate of Analysis	Oct 11, 2022	T117927
F2221526	Certificate of Analysis	Jun 12, 2022	T117927
F2221527	Certificate of Analysis	Jun 12, 2022	T117927

Show more⌵

Chemical and Physical Properties

Sensitivity	Light sensitive

Documents & Articles

Peroxidase Substrate Detection System

Categories: Technical articles

Tags:

HRP Immunoassay reagents and substrates Peroxidase TMB DAB ABTS Enzyme inhibitors and substrates Chromogenic substrates and chemiluminescence substrates

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

=

Concentration

x

Volume

x

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

x

Volume 1 (V1)

=

Concentration 2 (C2)

x

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

=

Mass (in vial)

÷

Desired Reconstitution Concentration

SKU	Size	Availability	Price
T117927-25ml	25ml	3	$52.90
T117927-100ml	100ml	5	$162.90
T117927-500ml	500ml	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$618.90

TMB color reagent A solution (3,3',5,5'-tetramethylbenzidine)