Skip to the beginning of the images gallery

TelN Protelomerase

COA

Grade & Purity:

for molecular biology

for DNA and RNA applications

10 U/μL

In stock

Item Number

T767021

Grouped product items
SKU	Size	Availability	Price	Qty
T767021-250U	250U	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$99.90
T767021-1000U	1000U	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$349.90

View related series

Endonuclease (5) Nuclease (7)

Basic Description

Product Name

TelN Protelomerase

Synonyms

TelN Protelomerase

Grade

BioReagent, for DNA and RNA applications, for DNA synthesis, for molecular biology, PCR Reagent, 废弃Ph. Eur., EnzymoPure™

Product Description

T767021	Component	250 U	1000 U	Storage
T767021A	TelN Protelomerase	25 μL	100 μL	-20°C. Avoid freeze/thaw cycle
T767021B	10×TelN Reaction Buffer	250 μL	1 mL	-20°C. Avoid freeze/thaw cycle

TelN Protelomerase, whose Chinese name is TelN Prokaryotic telomerase, is cloned from phage N15, which cuts dsDNA at the recognition site TelRL (56bp), The TelRL site consists of a palindromic sequence TelO in the middle and a palindromic sequence R3 and L3 at both ends of 14bp (Fig.1), and leaves a covalently closed end at the cleavage site, thus effectively converting circular DNA into linear DNA with hairpin ends (Fig.2).

Source
E.coli
Enzyme Activity Definition
The amount of enzyme required to completely cut 0.5 μg linearized pMiniT-TelN control plasmid (313 fmol TelN recognition site) for 30 min in a 50 μl system at 30°C was defined as 1 unit of activity (U).
Protocol
(1) Configure the mixture as shown in the following table:

Component	volume
dsDNA	X μl
10×TelN Reaction Buffer	2 μl
TelN Protelomerase	1 μl
Nuclease-free Water	To 20 μl

(2) Shake the mixed system gently up and down, and centrifuge briefly.
(3) Incubate at 30℃ for 30 min.
(4) Incubation at 75℃ for 5 min terminated the reaction.
Cautions
(1) This product is for scientific research purposes only and shall not be used for other purposes (since 2022.05.31).
(2) In some application scenarios, there are patent restrictions on the use of this enzyme.

Specifications & Purity

EnzymoPure™, BioReagent, for DNA synthesis, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology, For In Vitro Transcription, 10 U/μL

Storage and Shipping

Concentration	10 U/μL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

TelN Protelomerase

Basic Description

Storage and Shipping

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews