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Recombinant Streptavidin Protein (LumiDye™ Blue 525), CAS No.9013-20-1

1 Citations

COA

Grade & Purity:

Validated

ExactAb™

1.0 mg/mL

Bioactivity: Binding Biotin

In stock

Item Number

rp220555

Grouped product items
SKU	Size	Availability	Price
rp220555-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$10.90
rp220555-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$19.90
rp220555-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$99.90

Recombinant; Streptavidin (LumiDye™ Blue 525); Flow Cytometry, IF/ICC

View related series

LumiDye™ Blue 525 (4) SA (55) Streptavidin (58) Streptavidin Protein (37)

Basic Description

Product Name	Recombinant Streptavidin Protein (LumiDye™ Blue 525), CAS No.9013-20-1
Synonyms	SA V1 \| SA V2 \| strepavidin \| Streptavidin V1 \| Streptavidin V2 \| Streptavidin
Grade	ExactAb™, Validated
Product Description	Streptavidin is used as a tetrameric protein used to visualize biotin conjugated molecules in ELISA. Due to the strong affinity of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin is widely used in molecular biology through its unique high affinity for the biotin.
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Biochemical and Physiological Mechanisms	Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.
Bioactivity	Binding Biotin
Conjugation	废弃FITC. Ex: 493nm, Em: 528nm
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	525nm
Ex/Em(nm)

Storage and Shipping

Concentration	1.0 mg/mL
Storage Temp	Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
CAS	9013-20-1

Images

IF/ICC: Recombinant Streptavidin Protein (LumiDye™ Blue 525) (rp220555)

Recombinant Streptavidin Protein (LumiDye™ Blue 525) (rp220555) – IF/ICC
IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 4% PFA for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with biotinylated Oportuzumab (anti-EpCAM) (Ab190146) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (LumiDye™ Blue 525) (rp220555) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Recombinant Streptavidin Protein (LumiDye™ Blue 525) (rp220555)

Recombinant Streptavidin Protein (LumiDye™ Blue 525) (rp220555) - Flow Cytometry
Flow cytometry analysis of EpCAM expression by HT-29 cells (red) compared to an isotype control (blue). Cells were stained with biotinylated Oportuzumab (anti-EpCAM) (Ab190146) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (LumiDye™ Blue 525) (rp220555) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Human IgG (Biotin) (Ab180314). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
IF/ICC	1-10μg/mL
Flow Cytometry	0.5-10μg/mL

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