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Recombinant Streptavidin Protein (AF660), CAS No.9013-20-1

1 Citations

COA

Grade & Purity:

Validated

ExactAb™

1.0 mg/mL

Bioactivity: Binding Biotin

In stock

Item Number

rp177491

Grouped product items
SKU	Size	Availability	Price
rp177491-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$39.90
rp177491-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$129.90
rp177491-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$499.90

Recombinant; Streptavidin (AF660); Flow Cytometry, IF

View related series

AF660 (4) SA (55) Streptavidin (58) Streptavidin Protein (37)

Basic Description

Product Name	Recombinant Streptavidin Protein (AF660), CAS No.9013-20-1
Synonyms	SA V1 \| SA V2 \| strepavidin \| Streptavidin V1 \| Streptavidin V2 \| Streptavidin
Grade	ExactAb™, Validated
Product Description	Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Biochemical and Physiological Mechanisms	Streptavidin is a biotin-binding protein that binds four biotin molecules. The biotin-binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and polymerization.
Bioactivity	Binding Biotin
Conjugation	废弃PE. Ex: 488nm, Em: 575nm
Excitation(nm)	Red Laser(633nm)
Emission(nm)	691nm
Ex/Em(nm)

Storage and Shipping

Concentration	1.0 mg/mL
Storage Temp	Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
CAS	9013-20-1

Images

IF: Recombinant Streptavidin Protein (AF660) (rp177491)

Recombinant Streptavidin Protein (AF660) (rp177491) - IF
IF analysis of beta I Tubulin (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with biotinylated Recombinant beta I Tubulin Antibody (Ab091144) dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (AF660) (rp177491) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Recombinant Streptavidin Protein (AF660) (rp177491)

Recombinant Streptavidin Protein (AF660) (rp177491) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab185413) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (AF660) (rp177491) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
IF/ICC	1-10μg/mL
Flow Cytometry	0.5-10μg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

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Analytical Chart:

Citations of This Product

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