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Recombinant Kex2 Protease, CAS No.99676-46-7

Grade & Purity:

≥90%(SDS-PAGE)
≥10 units/mg

Accession #: P13134
Bioactivity: Specific Activity: ≥10U/mg pro. UNIT DEFINITION: Under the conditions of 25°C and pH 8.0, the amount of enzyme needed to catalyze the conversion of 1 μmol Boc-QR R-pNA into products per minute is one enzyme activity unit (U).
Endotoxin Concentration: <0.1 EU/μg

In stock

Item Number

R141093

Grouped product items
SKU	Size	Availability	Price
R141093-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$129.90
R141093-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$329.90
R141093-5mg	5mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$809.90
R141093-10mg	10mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,139.90

GMP, ≥90% (SDS-PAGE), Active, Pichia pastoris, No tag

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Accession#: P13134 (1) Amino Acid/Protein Metabolism (1836) Gene ID: 855483 (1) KEX2 (1)

Basic Description

Product Name	Recombinant Kex2 Protease, CAS No.99676-46-7
Synonyms	KEX 2 \| Kex2p \| Kexin \| KEX2 \| QDS1 \| SRB1 \| VMA45
Grade	ActiBioPure™, Bioactive, Carrier Free, GMP, EnzymoPure™
Product Description	Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α,factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃. It is stable in buffer (pH 5.0-6.0). The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition. Kex2 Protease Recommended Method: Recommended reaction buffer: 5-10 mM Tris, 2mM Ca2+, pH 7.0-9.0 or HEPES, 5mM Ca2+, pH 7.0-9.0. If it is not used immediately after dissolution, it is recommended to use 20mM NaAc-HAc, 2mM Ca2+ (pH 5.0~5.5) buffer to dissolve the lyophilized powder. During the reaction, use 5-10 mM Tris, 2mM Ca2+, pH 7.0-9.0 or HEPES, 5mM Ca2+, pH 7.0-9.0 as the reaction diluent. Recommended conditions: Protease: Fusion protein Ratio 1:20~1:1000.
Specifications & Purity	Carrier Free, GMP, Bioactive, ActiBioPure™, EnzymoPure™, ≥90%(SDS-PAGE), ≥10 units/mg
Biochemical and Physiological Mechanisms	Catalytic activity: Cleavage of -Lys-Arg-\|-Xaa- and -Arg-Arg-\|-Xaa- bonds to process yeast alpha-factor pheromone and killer toxin precursors. Cofactor Ca2+. Note: Binds 3 Ca2+ ions per subunit. Post-translational: O-glycosylated.
Bioactivity	Specific Activity: ≥10U/mg pro. UNIT DEFINITION: Under the conditions of 25°C and pH 8.0, the amount of enzyme needed to catalyze the conversion of 1 μmol Boc-QR R-pNA into products per minute is one enzyme activity unit (U).
Endotoxin Concentration	<0.1 EU/μg
Accession #	P13134
Predicted molecular weight	80 kDa
SDS-PAGE	68-92 kDa

Storage and Shipping

Concentration	≥10 units/mg
Reconstitution	20mM NaAc-HAc, 2mM Ca2+, pH 5.0~5.5.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Lyophilized powder store at -20℃ for 24 months. Dissolve in buffer (20mM NaAc-HAc, 2 mM Ca2+, pH 5.0~5.5), subpackage and store at -20℃. It is recommended that it should be stored at 2~8℃ after dissolution for no more than 24 hours. Upon receipt, it is re
CAS	99676-46-7
Enzyme Commission Number	EC: 3.4.21.61

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

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