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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab222885-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$10.90
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Ab222885-50μg
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50μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$26.90
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Ab222885-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$41.90
|
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Ab222885-1mg
|
1mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$299.90
|
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Recombinant (P97D04); Mouse anti Human IL-2 RA Antibody; Flow Cytometry, ELISA; Unconjugated
| Product Name | Recombinant IL-2 Receptor alpha/CD25 Antibody - Primary antibody, specific to Human IL-2 Receptor alpha |
|---|---|
| Specifications & Purity | Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), See COA |
| Biochemical and Physiological Mechanisms | Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
| Host species | Mouse |
| Specificity | Human IL-2 Receptor alpha |
| Immunogen | Recombinant Human CD25 |
| Positive Control | Flow Cytometry: PHA-stimulated (3 days) human peripheral blood lymphocytes |
| Conjugation | Unconjugated |
| Grade | Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated |
| Product Description |
Recombinant IL-2 Receptor alpha/CD25 Antibody, clone P97D04, could be used for Flow Cytometry, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant IL-2 Receptor alpha/CD25 Antibody (Ab222885) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 10 μg/mL PHA (L1668) stimulated (right) human peripheral blood lymphcytes labelling IL-2 Receptor alpha/CD25 (red) with Recombinant IL-2 Receptor alpha/CD25 Antibody (Ab222885) at 0.2 μg/mL for 1 hour at 4°C, and detected with Alexa Fluor 488-conjugated affinipure goat anti-Mouse IgG. Blue - Mouse IgG Isotype Control Antibody (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant IL-2 Receptor alpha/CD25 Antibody (Ab222885) - SEC
The purity of Recombinant IL-2 Receptor alpha/CD25 Antibody (Ab222885) is more than 95% verified by HPLC.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.5 µg per million cells in 100 µl volume |