Bioactivity:
1. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 5.0 μg/mL can bind Recombinant Human ACE-2 Protein (rp176242) with the EC50 of 75.79 ng/mL. 2. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Regdanvimab (anti-Spike RBD) (Ab182925) with the EC50 of 12.65 ng/mL. 3. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Imdevimab (anti-Spike RBD) (Ab182897) with the EC50 of 7.82 ng/mL. 4. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Etesevimab (anti-Spike RBD) (Ab182932) with the EC50 of 23.96 ng/mL. 5. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Sotrovimab (anti-Spike RBD) (Ab182938) with the EC50 of 41.33 ng/mL. 6. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Casirivimab (anti-Spike RBD) (Ab182896) with the EC50 of 10.50 ng/mL.
Endotoxin Concentration:
<1.0 EU/μg
In stock
Item Number
rp155929
Grouped product items
SKU
Size
Availability
Price
Qty
rp155929-10μg
10μg
≥10
$139.90
rp155929-100μg
100μg
9
$499.90
rp155929-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Specifications & Purity
Animal Free, Carrier Free, Bioactive, ActiBioPure™, ≥95%(SDS-PAGE), See COA
Purity
＞95% (SDS-PAGE)
Bioactivity
1. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 5.0 μg/mL can bind Recombinant Human ACE-2 Protein (rp176242) with the EC50 of 75.79 ng/mL. 2. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Regdanvimab (anti-Spike RBD) (Ab182925) with the EC50 of 12.65 ng/mL. 3. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Imdevimab (anti-Spike RBD) (Ab182897) with the EC50 of 7.82 ng/mL. 4. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Etesevimab (anti-Spike RBD) (Ab182932) with the EC50 of 23.96 ng/mL. 5. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Sotrovimab (anti-Spike RBD) (Ab182938) with the EC50 of 41.33 ng/mL. 6. Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Casirivimab (anti-Spike RBD) (Ab182896) with the EC50 of 10.50 ng/mL.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C for 1 year, store at 2-8℃ for 1 month. Avoid freeze / thaw cycle.
Images
Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) - SDS-PAGE 3 μg/lane of Recombinant Human SARS-CoV-2 Spike Protein RBD was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a band at 36.4 kDa under reducing conditions and 34.2 kDa under non-reducing conditions.
Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) - ELISA Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Regdanvimab (anti-Spike RBD) (Ab182925) with the EC₅₀ of 12.65 ng/mL.
Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) - ELISA Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Imdevimab (anti-Spike RBD) (Ab182897) with the EC₅₀ of 7.82 ng/mL.
Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) - ELISA Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Etesevimab (anti-Spike RBD) (Ab182932) with the EC₅₀ of 23.96 ng/mL.
Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) - ELISA Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Sotrovimab (anti-Spike RBD) (Ab182938) with the EC₅₀ of 41.33 ng/mL.
Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) - ELISA Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Casirivimab (anti-Spike RBD) (Ab182896) with the EC₅₀ of 10.50 ng/mL.
Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) - ELISA Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 1.0 μg/mL can bind Cilgavimab (anti-Spike RBD) (Ab182901) with the EC50 of 173.8 ng/mL.
Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) - Binding Activity Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) at 5.0 μg/mL can bind Recombinant Human ACE-2 Protein (rp176242) with the EC₅₀ of 75.79 ng/mL.
Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) - ELISA Immobilized Recombinant Human SARS-CoV-2 Spike Protein RBD protein (rp155929) at 1.0 μg/mL can bind Bebtelovimab (anti-Spike RBD) (Ab175465) with the EC
₅₀
of 32.26 ng/mL.
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
ZJ24F0202908