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Recombinant Human ROBO1/DUTT1 Protein

COA

Grade & Purity:

Animal Free

Carrier Free

≥95%(SDS-PAGE)

Expression System: HEK293
Accession #: Q9Y6N7-1
Protein Tag: C-His
Bioactivity: Testing in progress
Endotoxin Concentration: <1.0 EU/μg

In stock

Item Number

rp184133

Grouped product items
SKU	Size	Availability	Price
rp184133-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$139.90
rp184133-50μg	50μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$399.90
rp184133-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$639.90
rp184133-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$3,999.90

Animal Free, ≥95% (SDS-PAGE), 293F cell, C-His tag, 1-897 aa

View related series

Click to view protein information: ROBO1

Accession#: Q9Y6N7-1 (1) Gene ID:6091 (2) ROBO1 (2) ROBO1/DUTT1 (1)

Basic Description

Product Name	Recombinant Human ROBO1/DUTT1 Protein
Synonyms	DUTT1Drosophila) homolog 1 \| DUTT1 \| H-Robo-1 \| MGC131599 \| MGC133277 \| ROBO1 \| roundabout, axon guidance receptor, homolog 1 (Drosophila)
Grade	Animal Free, Carrier Free
Product Description	Protein Function: Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development (PubMed:10102268, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257). May be required for lung development (By similarity).
Specifications & Purity	Animal Free, Carrier Free, ≥95%(SDS-PAGE)
Bioactivity	Testing in progress
Endotoxin Concentration	<1.0 EU/μg
Expression System	HEK293
Amino Acids	1-897 aa
Sequence	MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPHHHHHH
Protein Tag	C-His
Accession #	Q9Y6N7-1
Predicted molecular weight	99.3 kDa
SDS-PAGE	114.9 kDa, under reducing conditions; 111.5 kDa, under non-reducing conditions

Storage and Shipping

Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

SDS-PAGE: Recombinant Human ROBO1/DUTT1 Protein (rp184133)

Recombinant Human ROBO1/DUTT1 Protein (rp184133) - SDS-PAGE
3 μg/lane of Recombinant Human ROBO1/DUTT1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 114.9 kDa under reducing conditions and 111.5 kDa under non-reducing conditions.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

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Analytical Chart:

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0708956	Certificate of Analysis	Aug 02, 2024	rp184133
ZJ24R0700816	Certificate of Analysis	Jul 21, 2024	rp184133

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