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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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rp181310-10μg
|
10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$89.90
|
|
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rp181310-50μg
|
50μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$269.90
|
|
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rp181310-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$429.90
|
|
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rp181310-1mg
|
1mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$3,399.90
|
|
Animal Free, ≥95% (SDS-PAGE), Active, HEK293, C-His, 1-201 aa
| Product Name | Recombinant Human RBP4 Protein |
|---|---|
| Synonyms | interstitial | Plasma retinol-binding protein | RBP4 | retinol binding protein 4, plasma | RetinolBinding Protein 4 | retinol-binding protein 4, plasma |
| Grade | ActiBioPure™, Animal Free, Bioactive, Carrier Free |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE) |
| Biochemical and Physiological Mechanisms | Retinol (also known as vitamin A) is unstable and insoluble in aqueous solutions. However, retinol becomes quite stable and soluble in plasma due to its tight interaction with retinol-binding protein 4 (RBP4), also known as plasma retinol-binding protein. |
| Bioactivity | 1. Immobilized Recombinant Human RBP4 Protein (rp181310) at 1.0 μg/mL can bind RBP4 Mouse mAb (Ab176170) with the EC50 of 20.2 ng/mL. 2. Immobilized Recombinant Human RBP4 Protein (rp181310) at 1.0 μg/mL can bind RBP4 Mouse mAb (Ab176167) with the EC50 of 73.4 ng/mL. 3. Immobilized Recombinant Human RBP4 Protein (rp181310) at 1.0 μg/mL can bind RBP4 Mouse mAb (Ab176165) with the EC50 of 61.7 ng/mL. |
| Endotoxin Concentration | <1.0 EU/μg |
| Amino Acids | 1-201 aa |
| Sequence | MKWVWALLLLAALGSGRAERDCRVSSFRVKENFDKARFSGTWYAMAKKDPEGLFLQDNIVAEFSVDETGQMSATAKGRVRLLNNWDVCADMVGTFTDTEDPAKFKMKYWGVASFLQKGNDDHWIVDTDYDTYAVQYSCRLLNLDGTCADSYSFVFSRDPNGLPPEAQKIVRQRQEELCLARQYRLIVHNGYCDGRSERNLLHHHHHH |
| Accession # | P02753 |
| Predicted molecular weight | 21.9 kDa |
| SDS-PAGE | 18.8-24.1 kDa, under reducing conditions; 17.6 & 38.8 kDa, under non-reducing conditions. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
|---|---|
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C for 1 year. Avoid freeze/thaw cycle. |
Recombinant Human RBP4 Protein (rp181310) - ELISA
Immobilized Recombinant Human RBP4 Protein (rp181310) at 1.0 μg/mL can bind RBP4 Mouse mAb (Ab176170) with the EC50 of 20.2 ng/mL.
Recombinant Human RBP4 Protein (rp181310) - ELISA
Immobilized Recombinant Human RBP4 Protein (rp181310) at 1.0 μg/mL can bind RBP4 Mouse mAb (Ab176167) with the EC50 of 73.4 ng/mL.
Recombinant Human RBP4 Protein (rp181310) - ELISA
Immobilized Recombinant Human RBP4 Protein (rp181310) at 1.0 μg/mL can bind RBP4 Mouse mAb (Ab176165) with the EC50 of 61.7 ng/mL.
Recombinant Human RBP4 Protein (rp181310) - SDS-PAGE
3 μg/lane of Recombinant Human RBP4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 18.8-24.1 kDa under reducing conditions and 17.6 & 38.8 kDa under non-reducing conditions.