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Recombinant Human PD-L1 Protein

  • Expression System: HEK293
  • Accession #: Q9NZQ7
  • Protein Tag: C-His
  • Bioactivity: Immobilized Recombinant Human PD-L1 Protein (rp169666) at 2.0 μg/mL can bind Sugemalimab (anti-PD-L1) (Ab175662) with the EC50 of 169.70 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Avelumab (anti-PD-L1) (A412006) with the EC50 of 77.96 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Envafolimab (anti-PD-L1) (Ab170617) with the EC50 of 339.40 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Durvalumab (anti-PD-L1) (D412005) with the EC50 of 35.40 ng/mL.
  • Endotoxin Concentration: <1.0 EU/μg
In stock
Item Number
rp169666
Grouped product items
SKU Size
Availability
Price Qty
rp169666-10μg
10μg
≥10
$139.90
rp169666-50μg
50μg
10
$299.90
rp169666-100μg
100μg
10
$399.90
rp169666-1mg
1mg
1
$2,999.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F cell, C-His tag, 1-239 aa

Basic Description

Product Name Recombinant Human PD-L1 Protein
Synonyms B7 H | B7 H1 | B7 homolog 1 | B7-H1 | B7H | B7H1 | CD 274 | CD-274 | CD274 | CD274 antigen | CD274 molecule | MGC142294 | MGC142296 | OTTHUMP00000021029 | PD L1 | PD-L1 | PD1L1_HUMAN | PDCD1 ligand 1 | PDCD1L1 | PDCD1LG1 | PDL 1 | PDL1 | Programmed cell d
Grade Animal Free, Bioactive, Carrier Free
Product Description

Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description:
Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Specifications & Purity Animal Free, Carrier Free, Bioactive, ≥95%(SDS-PAGE)
Bioactivity Immobilized Recombinant Human PD-L1 Protein (rp169666) at 2.0 μg/mL can bind Sugemalimab (anti-PD-L1) (Ab175662) with the EC50 of 169.70 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Avelumab (anti-PD-L1) (A412006) with the EC50 of 77.96 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Envafolimab (anti-PD-L1) (Ab170617) with the EC50 of 339.40 ng/mL. Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Durvalumab (anti-PD-L1) (D412005) with the EC50 of 35.40 ng/mL.
Endotoxin Concentration <1.0 EU/μg
Expression System HEK293
Amino Acids 1-239 aa
Sequence MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHHHHHH
Protein Tag C-His
Accession # Q9NZQ7
Predicted molecular weight 26.1 kDa
SDS-PAGE 30.7-37.3 kDa, under reducing conditions; 28.6-36.2 kDa, under non-reducing conditions.

Storage and Shipping

Shape Lyophilized
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

Recombinant Human PD-L1 Protein (rp169666) - ELISA
Immobilized Recombinant Human PD-L1 Protein (rp169666) at 2.0 μg/mL can bind Sugemalimab (anti-PD-L1) (Ab175662) with the EC₅₀ of 169.70 ng/mL.


Recombinant Human PD-L1 Protein (rp169666) - ELISA
Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Avelumab (anti-PD-L1) (A412006) with the EC₅₀ of 77.96 ng/mL.


Recombinant Human PD-L1 Protein (rp169666) - ELISA
Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Envafolimab (anti-PD-L1) (Ab170617) with the EC₅₀ of 339.40 ng/mL.

Recombinant Human PD-L1 Protein (rp169666) - ELISA
Immobilized Recombinant Human PD-L1 Protein (rp169666) at 1.0 μg/mL can bind Durvalumab (anti-PD-L1) (D412005) with the EC₅₀ of 35.40 ng/mL.

Recombinant Human PD-L1 Protein (rp169666) - SDS-PAGE
3 μg/lane of Recombinant Human PD-L1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.7-37.3 kDa under reducing conditions and 28.6-36.2 kDa under non-reducing conditions.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24F0606445 Certificate of Analysis Jun 07, 2024 rp169666
ZJ24F0606444 Certificate of Analysis Jun 07, 2024 rp169666
ZJ24F0606443 Certificate of Analysis Jun 07, 2024 rp169666
ZJ24F0606442 Certificate of Analysis Jun 07, 2024 rp169666

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