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Recombinant Human Osteoprotegerin Protein, ＞95%(SDS-PAGE, HPLC), high purity

Grade & Purity:

≥95%(SDS-PAGE)

Expression System: E.coli
Accession #: O00300
Protein Tag: No tag
Bioactivity: Fully biologically active when compared to standard. The ED50 as determined by neutralizing the stimulation of U937 cells is less than 10 ng/ml in the presence of 10 ng/mL soluble rHuRANKL (sRANKL).
Endotoxin Concentration: <1.0 EU/μg

In stock

Item Number

rp149600

Grouped product items
SKU	Size	Availability	Price
rp149600-10μg	10μg	6	$59.90
rp149600-50μg	50μg	3	$139.90
rp149600-100μg	100μg	1	$199.90
rp149600-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,799.90

Animal Free, >95%(SDS-PAGE, HPLC), Active, E.coli, No tag, 22-194 aa

View related series

Click to view protein information: TNFRSF11B

Accession#:O00300 (1) Gene ID:4982 (1) Osteoprotegerin (1) TNFRSF11B (1)

Basic Description

Product Name	Recombinant Human Osteoprotegerin Protein, ＞95%(SDS-PAGE, HPLC), high purity
Synonyms	OCIF \| OCIFMGC29565 \| OPG \| OPGtumor necrosis factor receptor superfamily member 11B \| Osteoclastogenesis inhibitory factor \| Osteoprotegerin \| TNFRSF11B \| TR1 \| tumor necrosis factor receptor superfamily, member 11b \| PDB5
Grade	ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description	Purity > 95 % by SDS-PAGE and HPLC analyses. Function Osteoprotegerin (OPG), also named osteoclastogenesis inhibitory factor (OCIF), and tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), is a TNFRSF11B-encoded protein in humans. Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.OPG has been applied to decrease bone resorption in women with postmenopausal osteoporosis and in patients with lytic bone metastases.
Specifications & Purity	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Purity	＞95%(SDS-PAGE, HPLC)
Bioactivity	Fully biologically active when compared to standard. The ED50 as determined by neutralizing the stimulation of U937 cells is less than 10 ng/ml in the presence of 10 ng/mL soluble rHuRANKL (sRANKL).
Endotoxin Concentration	<1.0 EU/μg
Expression System	E.coli
Species	Human
Amino Acids	22-194 aa
Sequence	ETFPPKYLHY DEETSHQLLC DKCPPGTYLK QHCTAKWKTV CAPCPDHYYT DSWHTSDECL YCSPVCKELQ YVKQECNRTH NRVCECKEGR YLEIEFCLKH RSCPPGFGVV QAGTPERNTV CKRCPDGFFS NETSSKAPCR KHTNCSVFGL LLTQKGNATH DNICSGNSES TQK
Protein Tag	No tag
Accession #	O00300
Predicted molecular weight	19.7 kDa

Storage and Shipping

Shape	Lyophilized
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ℃ as supplied; 1 month, 2 to 8 ℃ under sterile conditions after reconstitution; 3 months, -20 to -70 ℃under sterile conditions after reconstitut

Images

Protein Bioactivity: Recombinant Human Osteoprotegerin Protein (rp149600)

Recombinant Human Osteoprotegerin Protein (rp149600)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by neutralizing the stimulation of U937 cells is less than 10 ng/mL, corresponding to a specific activity of > 1.0 ×10⁵ IU/mg in the presence of 10 ng/mL soluble rHuRANKL (sRANKL).

SDS-PAGE: Recombinant Human Osteoprotegerin Protein (rp149600)

Recombinant Human Osteoprotegerin Protein (rp149600)-SDS-PAGE
Recombinant Human Osteoprotegerin Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a single band at 20.7 kDa.

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3 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0600344	Certificate of Analysis	Mar 26, 2024	rp149600
ZJ23F0600345	Certificate of Analysis	Mar 26, 2024	rp149600
ZJ23F0600346	Certificate of Analysis	Jun 25, 2023	rp149600

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