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Recombinant Human Nectin-4 Protein

Grade & Purity:

≥95%(SDS-PAGE)

Expression System: HEK293
Accession #: Q96NY8-1
Protein Tag: C-His
Bioactivity: Immobilized Recombinant Human Nectin-4 Protein (rp183631) at 1.0 μg/mL can bind Enfortumab vedotin-ejfv (anti-Nectin-4) (Ab175528) with the EC50 of 17.59 ng/mL.
Endotoxin Concentration: <1.0 EU/μg

In stock

Item Number

rp183631

Grouped product items
SKU	Size	Availability	Price
rp183631-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$139.90
rp183631-50μg	50μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$399.90
rp183631-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$639.90
rp183631-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,999.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F cell, C-His tag, 1-349 aa

View related series

Click to view protein information: NECTIN4

Accession#: Q96NY8-1 (1) Gene ID:81607 (3) Nectin-4 (2) NECTIN4 (3)

Basic Description

Product Name	Recombinant Human Nectin-4 Protein
Synonyms	Ig superfamily receptor LNIR \| EDSS1 \| LNIR \| LNIRnectin 4 \| Nectin4 \| Nectin-4 \| poliovirus receptor-related 4 \| poliovirus receptor-related protein 4 \| PRR4 \| PRR4DKFZp686K05193 \| PVRL4
Grade	ActiBioPure™, Animal Free, Bioactive, Carrier Free
Product Description	Protein Function:： Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. The Latin word necto means “to connect”, indicating the role of nectins in Ca2+‑independent cell-cell adhesion. Nectin-4 forms homodimers in cis, followed by interactions in trans with Nectin-1 or -4. Human Nectin-4 mRNA is normally expressed in the placenta, especially in endothelial cells, while in the mouse it is found in the embryo, lung, testis and brain. Human Nectin-4 cDNA encodes 510 amino acids (aa), including a 31 aa signal sequence, a 318 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 140 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N‑terminal V-type that mediates ligand binding, and two C2-type. One Nectin‑4 isoform lacks aa 412-436 in the cytoplasmic domain. In many human ductal breast or non-small cell lung carcinomas, Nectin‑4 is upregulated and a soluble 43 kDa form is found in the plasma . This form is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 91%, 92%, 93%, 91% and 90% amino acid sequence homology with the corresponding regions of mouse, rat, canine, porcine and bovine Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins. These interactions organize the actin cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. In humans, mutation of Nectin-4 has been correlated with ectodermal dysplasia-syndactyly syndrome, indicating a role for Nectin-4 in human development.
Specifications & Purity	ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE)
Bioactivity	Immobilized Recombinant Human Nectin-4 Protein (rp183631) at 1.0 μg/mL can bind Enfortumab vedotin-ejfv (anti-Nectin-4) (Ab175528) with the EC50 of 17.59 ng/mL.
Endotoxin Concentration	<1.0 EU/μg
Expression System	HEK293
Amino Acids	1-349 aa
Sequence	MPLSLGAEMWGPEAWLLLLLLLASFTGRCPAGELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASHHHHHH
Protein Tag	C-His
Accession #	Q96NY8-1
Predicted molecular weight	34.9 kDa
SDS-PAGE	39.8 kDa, under reducing conditions; 37.4 kDa, under non-reducing conditions

Storage and Shipping

Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

ELISA: Recombinant Human Nectin-4 Protein (rp183631)

Recombinant Human Nectin-4 Protein (rp183631) - ELISA
Immobilized Recombinant Human Nectin-4 Protein (rp183631) at 1.0 μg/mL can bind Enfortumab vedotin-ejfv (anti-Nectin-4) (Ab175528) with the EC₅₀ of 17.59 ng/mL.

SDS-PAGE: Recombinant Human Nectin-4 Protein (rp183631)

Recombinant Human Nectin-4 Protein (rp183631) - SDS-PAGE
3 μg/lane of Recombinant Human Nectin-4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 39.8 kDa under reducing conditions and 37.4 kDa under non-reducing conditions.

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Analytical Chart:

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Lot Number	Certificate Type	Date	Item
ZJ24R0700842	Certificate of Analysis	Jul 26, 2024	rp183631

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