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Recombinant Human Mesothelin Protein

Grade & Purity:

≥95%(SDS-PAGE)

Expression System: HEK293
Accession #: Q13421
Protein Tag: C-His
Bioactivity: Measured by its binding ability in a functional ELISA. Human Mesothelin protein (rp176301) at 1 μg/mL can bind Human Osteopontin ( rp149597) with the EC50 of 30.0 ng/mL.
Endotoxin Concentration: <1.0 EU/μg

In stock

Item Number

rp176301

Grouped product items
SKU	Size	Availability	Price
rp176301-10μg	10μg	≥10	$69.90
rp176301-50μg	50μg	10	$199.90
rp176301-100μg	100μg	10	$319.90
rp176301-1mg	1mg	1	$2,599.90

Animal Free, >95% (SDS-PAGE), Active, 293F cell, His tag, 1-286 aa

View related series

Click to view protein information: MSLN

Accession#:Q13421 (6) Gene ID:10232 (6) Mesothelin (7) MSLN (7)

Basic Description

Product Name	Recombinant Human Mesothelin Protein
Synonyms	CAK 1 \| CAK1 \| CAK1 antigen \| cleaved form \| Megakaryocyte potentiating factor \| Mesothelin \| Mesothelin isoform 1 precursor \| MPF \| MPFSMRP \| MSLN \| MSLN_HUMAN \| Pre pro megakaryocyte potentiating factor \| Pre-pro-megakaryocyte-potentiating factor \| SMR
Grade	ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description	Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Mesothelin (MSLN), also known as CAK1 and ERC, is a glycosylated cell-surface antigen present on normal mesothelial cells and over-expressed in several human tumors. The mesothelin gene encodes a ~70 kDa precursor protein that is cleaved at a dibasic proteolytic site into a 40 kDa membrane-bound protein termed MSLN and a 31 kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Cleaved, human MSLN remains attached to the cell surface via a GPI linkage and shares 58% amino acid sequence identity with mouse and rat MSLN. In human, alternate splicing generates additional MSLN isoforms that have either an eight amino acid insertion following Ser408 or a substituted C‑terminal region with no GPI anchor. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is up‑regulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression. It is co‑expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion. A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions.
Specifications & Purity	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Bioactivity	Measured by its binding ability in a functional ELISA. Human Mesothelin protein (rp176301) at 1 μg/mL can bind Human Osteopontin ( rp149597) with the EC50 of 30.0 ng/mL.
Endotoxin Concentration	<1.0 EU/μg
Expression System	HEK293
Amino Acids	1-286 aa
Sequence	MALPTARPLLGSCGTPALGSLLFLLFSLGWVQPSRTLAGETGQEAAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLRCLAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQRLLPAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQDQQEAARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSSRDPSWRQPERhhhhhh
Protein Tag	C-His
Accession #	Q13421
Predicted molecular weight	27.5 kDa
SDS-PAGE	30.3 kDa, reducing conditions; 30.3 kDa, non-reducing conditions

Storage and Shipping

Shape	Lyophilized
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

ELISA: Recombinant Human Mesothelin Protein (rp176301)

Recombinant Human Mesothelin Protein (rp176301) - ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Human Osteopontin (rp149597) is immobilized at 0.5 µg/mL, biotinylated Recombinant Human Mesothelin (rp176301) binds with an ED₅₀ of 30.73 ng/mL.

SDS-PAGE: Recombinant Human Mesothelin Protein (rp176301)

Recombinant Human Mesothelin Protein (rp176301) - SDS-PAGE
3 μg/lane of Recombinant Mesothelin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.3 kDa.

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5 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0303047	Certificate of Analysis	Mar 08, 2024	rp176301
ZJ24F0303046	Certificate of Analysis	Mar 08, 2024	rp176301
ZJ24F0303044	Certificate of Analysis	Mar 08, 2024	rp176301
ZJ24F0303045	Certificate of Analysis	Mar 08, 2024	rp176301
ZJ24R0200296	Certificate of Analysis	Feb 29, 2024	rp176301

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