Skip to the beginning of the images gallery

Recombinant Human Meprin alpha Subunit/MEP1A Protein

Grade & Purity:

≥95%(SDS-PAGE)

Expression System: HEK293
Accession #: Q16819
Protein Tag: C-10His
Bioactivity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH. The specific activity is >500 pmol/min/µg, as measured under the described conditions.
Endotoxin Concentration: <0.1 EU/μg

In stock

Item Number

rp148696

Grouped product items
SKU	Size	Availability	Price
rp148696-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$219.90
rp148696-50μg	50μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$659.90
rp148696-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,059.90
rp148696-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$5,299.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F, C-10*His tag, 22-601 aa & 27-601 aa

View related series

Click to view protein information: MEP1A

Accession#: Q16819 (1) Gene ID: 4224 (1) MEP1A (1) Meprin alpha (1)

Basic Description

Product Name	Recombinant Human Meprin alpha Subunit/MEP1A Protein
Synonyms	EC 3.4.24.18 \| Endopeptidase-2 \| N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha \| PABA peptide hydrolase \| PPH alpha \| bA268F1.1 (meprin A alpha (PABA peptide hydrolase)) \| EC 3.4.24 \| EC 3.4.24.18 \| endopeptidase-2 \| MEP1A \| meprin A su
Grade	ActiBioPure™, Animal Free, Bioactive, Carrier Free
Product Description	Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases. Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Meprin B (composed of beta subunits only). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity. The 746 amino acid sequence of human meprin alpha subunit precursor consists of a signal peptide (residues 1 to 21), a pro region (residues 22 to 65), and a mature chain (residues 66 to 746) containing following domains, catalytic (residues 62 to 263), MAM (residues 264 to 433), MATH (residues 434 to 593), EGF-like (residues 670 to 710), transmembrane (residues 713 to 740), and cytoplasmic (residues 741 to 746). The pro enzyme terminating at residue 601 was expressed and the secreted protein purified from conditioned medium. The molecular masses of recombinant human MEP1A are similar to those observed for the alpha subunit of rat Meprin A.
Specifications & Purity	ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE)
Bioactivity	Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH. The specific activity is >500 pmol/min/µg, as measured under the described conditions.
Endotoxin Concentration	<0.1 EU/μg
Expression System	HEK293
Species	Human
Amino Acids	22-601 aa & 27-601 aa
Sequence	VPIKYLPEENVHDADFGEQKDISEINLAAGLDLFQGDILLQKSRNGLRDPNTRWTFPIPYILADNLGLNAKGAILYAFEMFRLKSCVDFKPYEGESSYIIFQQFDGCWSEVGDQHVGQNISIGQGCAYKAIIEHEILHALGFYHEQSRTDRDDYVNIWWDQILSGYQHNFDTYDDSLITDLNTPYDYESLMHYQPFSFNKNASVPTITAKIPEFNSIIGQRLDFSAIDLERLNRMYNCTTTHTLLDHCTFEKANICGMIQGTRDDTDWAHQDSAQAGEVDHTLLGQCTGAGYFMQFSTSSGSAEEAALLESRILYPKRKQQCLQFFYKMTGSPSDRLVVWVRRDDSTGNVRKLVKVQTFQGDDDHNWKIAHVVLKEEQKFRYLFQGTKGDPQNSTGGIYLDDITLTETPCPTGVWTVRNFSQVLENTSKGDKLQSPRFYNSEGYGFGVTLYPNSRESSGYLRLAFHVCSGENDAILEWPVENRQVIITILDQEPDVRNRMSSSMVFTTSKSHTSPAINDTVIWDRPSRVGTYHTDCNCFRSIDLGWSGFISHQMLKRRSFLKNDDLIIFVDFEDITHLSQHHHHHHHHHH
Protein Tag	C-10His
Accession #	Q16819
Predicted molecular weight	67 kDa
SDS-PAGE	86 kDa under reducing conditions.

Storage and Shipping

Shape	Lyophilized
Reconstitution	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

SDS-PAGE: Recombinant Human Meprin alpha Subunit/MEP1A Protein (rp148696)

Recombinant Human Meprin alpha Subunit/MEP1A Protein (rp148696) - SDS-PAGE
2 μg/lane of Recombinant Human Meprin alpha Subunit/MEP1A Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 76 - 90 kDa under reducing condition and 180 - 248 kDa under non-reducing condition.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration