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Recombinant Human HGF GMP Protein

Grade & Purity:

≥95%(SDS-PAGE&SEC-HPLC)

Expression System: HEK293
Accession #: P14210-1
Bioactivity: Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is equal or less than 4.00 ng/mL. The specific activity of recombinant human HGF is >1.0 x 10^6 IU/mg, which is calibrated against the human HGF WHO International Standard (NIBSC code: 96/564).
Endotoxin Concentration: <0.1 EU/μg

In stock

Item Number

rp168049-GMP

Grouped product items
SKU	Size	Availability	Price	Qty
rp168049-GMP-25μg	25μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,569.90

GMP, ≥95% (SDS-PAGE&SEC-HPLC), Active, HEK293, C-His tag, 32-728 aa (D384N, D416N, L622S, H645R, V678I)

View related series

Click to view protein information: HGF

Accession#:P14210 (6) Gene ID:3082 (6) HGF (7)

Basic Description

Product Name	Recombinant Human HGF GMP Protein
Synonyms	Hepatocyte Growth Factor \| deafness, autosomal recessive 39 \| DFNB39 \| EC 3.4.21 \| EC 3.4.21.7 \| fibroblast-derived tumor cytotoxic factor \| F-TCF \| hepatocyte growth factor (hepapoietin A \| scatter factor) \| Hepatopoeitin-A \| Hepatopoietin A \| HGF \| HGFB
Grade	ActiBioPure™, Animal Free, Bioactive, Carrier Free, GMP, High Performance
Specifications & Purity	Animal Free, Carrier Free, GMP, ActiBioPure™, Bioactive, High performance, ≥95%(SDS-PAGE&SEC-HPLC)
Biochemical and Physiological Mechanisms	Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting i
Bioactivity	Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is equal or less than 4.00 ng/mL. The specific activity of recombinant human HGF is >1.0 x 10^6 IU/mg, which is calibrated against the human HGF WHO International Standard (NIBSC code: 96/564).
Endotoxin Concentration	<0.1 EU/μg
Expression System	HEK293
Amino Acids	32-728 aa (D384N, D416N, L622S, H645R, V678I)
Sequence	QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCNMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWNKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGSINYDGLLRVAHLYIMGNEKCSQRHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS
N-terminal Sequence	Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu (α-chain) Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn (β-chain)
Accession #	P14210-1
Predicted molecular weight	α chain: 53.7 kDa, β chain: 26 kDa
SDS-PAGE	71-85 kDa, non-reducing conditions. 56-65 kDa and 30-40 kDa, reducing conditions.

Storage and Shipping

Reconstitution	Reconstitute at 100 μg/mL in PBS.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	A minimum of 12 months when stored at ≤ -20 ℃ as supplied. 1 month, 2 to 8 ℃ under sterile conditions after reconstitution. 3 months, ≤ -20 ℃ under sterile conditions after reconstitution. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycl

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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