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Recombinant Human GDNF GMP Protein

  • Expression System: HEK293
  • Accession #: P39905-1
  • Protein Tag: No tag
  • Bioactivity: Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Human GFRα‑1/GDNF Rα‑1 Fc Chimera. The specific activity of recombinant human GDNF is >5.0 x 10^5 units/mg, which is calibrated against the human GDNF Reference Standard (NIBSC code: 09/266). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFRα‑1/GDNF Rα‑1 Fc Chimera at 1 µg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.
  • Endotoxin Concentration: <1.0 EU/μg
In stock
Item Number
rp168357-GMP
Grouped product items
SKU Size
Availability
Price Qty
rp168357-GMP-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$549.90
rp168357-GMP-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,829.90
rp168357-GMP-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$14,639.90

GMP, ≥97% (SDS-PAGE&SEC-HPLC), Active, HEK293, No tag, 109-211 aa

Basic Description

Product Name Recombinant Human GDNF GMP Protein
Synonyms Glial Cell line-derived Growth Factor | Astrocyte-derived trophic factor | ATF | ATF1 | ATF2 | GDNF | glial cell derived neurotrophic factor | glial cell line derived neurotrophic factor | glial cell line-derived neurotrophic factor | HFB1-GDNF | HGDNF |
Grade ActiBioPure™, Animal Free, Bioactive, Carrier Free, GMP, High Performance
Specifications & Purity ActiBioPure™, Bioactive, GMP, Carrier Free, Animal Free, High performance, ≥97%(SDS-PAGE&SEC-HPLC)
Biochemical and Physiological Mechanisms Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET rece
Bioactivity Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Human GFRα‑1/GDNF Rα‑1 Fc Chimera. The specific activity of recombinant human GDNF is >5.0 x 10^5 units/mg, which is calibrated against the human GDNF Reference Standard (NIBSC code: 09/266). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFRα‑1/GDNF Rα‑1 Fc Chimera at 1 µg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.
Endotoxin Concentration <1.0 EU/μg
Expression System HEK293
Amino Acids 109-211 aa
Sequence RGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI
Protein Tag No tag
N-terminal Sequence -Arg109-Gly-Gln-Arg-Gly-Lys-Asn-Arg-Gly-(Cys)
Accession # P39905-1
Predicted molecular weight 11.6 kDa (monomer)
SDS-PAGE 17 kDa, reducing conditions. 33 kDa, non-reducing conditions.

Storage and Shipping

Reconstitution Reconstitute at 100 μg/mL in PBS.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage A minimum of 12 months when stored at ≤ -20 ℃ as supplied. 1 month, 2 to 8 ℃ under sterile conditions after reconstitution. 3 months, ≤ -20 ℃ under sterile conditions after reconstitution. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycl

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

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