Skip to the beginning of the images gallery

Recombinant Human ErbB2/HER2 Protein, >90% (SDS-PAGE), high purity

Grade & Purity:

≥90%(SDS-PAGE)

Expression System: HEK293
Accession #: P04626
Protein Tag: C-His
Bioactivity: Pertuzumab (anti-HER2) (P412000) at 1.0 ug/mL can bind biotinylated Human ErbB2/HER2 Protein (rp169607), The EC50 for this effect is 25.99 ng/mL.
Endotoxin Concentration: <1.0 EU/μg

In stock

Item Number

rp169607

Grouped product items
SKU	Size	Availability	Price
rp169607-10μg	10μg	≥10	$99.90
rp169607-50μg	50μg	4	$369.90
rp169607-100μg	100μg	1	$599.90
rp169607-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$4,999.90

Animal Free, >90% (SDS-PAGE), Active, 293F cell, C-His tag, 1-652 aa

View related series

Click to view protein information: ERBB2

Accession#:P04626 (13) ERBB2 (13) ErbB2/HER2 (1) Gene ID:2064 (13)

Basic Description

Product Name	Recombinant Human ErbB2/HER2 Protein, >90% (SDS-PAGE), high purity
Synonyms	CD340 antigen \| CD340 \| c-erb B2/neu protein \| EC 2.7.10 \| EGFR2 \| ErbB2 \| HER2 \| HER-2 \| HER2EC 2.7.10.1 \| herstatin \| Metastatic lymph node gene 19 protein \| MLN 19 \| MLN19 \| Neu Oncogene \| NEUHER-2/neu \| neuroblastoma/glioblastoma derived oncogene homo
Grade	ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description	Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation.
Specifications & Purity	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥90%(SDS-PAGE)
Purity	>90% (SDS-PAGE)
Bioactivity	Pertuzumab (anti-HER2) (P412000) at 1.0 ug/mL can bind biotinylated Human ErbB2/HER2 Protein (rp169607), The EC50 for this effect is 25.99 ng/mL.
Endotoxin Concentration	<1.0 EU/μg
Expression System	HEK293
Amino Acids	1-652 aa
Sequence	MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTHHHHHH
Protein Tag	C-His
Accession #	P04626
Predicted molecular weight	70.2 kDa
SDS-PAGE	81.0-109.6 kDa, under reducing conditions

Storage and Shipping

Shape	Lyophilized
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

Protein Bioactivity: Recombinant Human ErbB2/HER2 Protein (rp169607)

Recombinant Human ErbB2/HER2 Protein (rp169607) - Protein Bioactivity
Pertuzumab (anti-HER2) (P412000) at 1.0 ug/ml can bind biotinylated Human ErbB2/HER2 Protein (rp169607), The EC₅₀ for this effect is 25.99 ng/mL.

SDS-PAGE: Recombinant Human ErbB2/HER2 Protein (rp169607)

Recombinant Human ErbB2/HER2 Protein (rp169607) - SDS-PAGE
3 μg/lane of Recombinant Human ErbB2/HER2 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 81.0-109.6 kDa under reducing conditions.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0303504	Certificate of Analysis	Mar 22, 2024	rp169607
ZJ24F0303505	Certificate of Analysis	Mar 22, 2024	rp169607
ZJ24F0303506	Certificate of Analysis	Mar 22, 2024	rp169607
ZJ24R0300334	Certificate of Analysis	Mar 15, 2024	rp169607

Documents & Articles

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration