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Recombinant Human DLL1 Protein, >95% (SDS-PAGE), high purity

  • Expression System: HEK293
  • Accession #: O00548
  • Protein Tag: C-His
  • Bioactivity: 1. Measured by its ability to bind human NOTCH1 in a functional ELISA. 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-20 μg/mL.
  • Endotoxin Concentration: <1.0 EU/μg
In stock
Item Number
rp145162
Grouped product items
SKU Size
Availability
Price Qty
rp145162-10μg
10μg
6
$129.90
rp145162-50μg
50μg
4
$359.90
rp145162-100μg
100μg
2
$619.90
rp145162-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,079.90

Animal Free, >95% (SDS-PAGE), Active, HEK293, C-His tag, 22-540 aa

Basic Description

Product Name Recombinant Human DLL1 Protein, >95% (SDS-PAGE), high purity
Synonyms delta (Drosophila)-like 1 | Delta 1 | Delta | Delta1 | delta-like 1 (Drosophila) | delta-like protein 1 | DL1 | DLL1 | Drosophila Delta homolog 1 | H-Delta-1 | NEDBAS | Delta1 | Drosophila Delta homolog 1 | H-Delta-1
Grade ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity
>95% (SDS-PAGE)
Endotoxin level
<1.0 EU/μg
Function
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
Post-translational
Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.

Specifications & Purity ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Purity >95% (SDS-PAGE)
Bioactivity 1. Measured by its ability to bind human NOTCH1 in a functional ELISA. 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-20 μg/mL.
Endotoxin Concentration <1.0 EU/μg
Expression System HEK293
Species Human
Amino Acids 22-540 aa
Sequence SGVFELKLQEFVNKKGLLGNRNCCRGGAGPPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGGGADSAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLATQRHLTVGEEWSQDLHSSGRTDLKYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGERGEKVCNPGWKGPYCTEPICLPGCDEQHGFCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWGGLFCNQDLNYCTHHKPCKNGATCTNTGQGSYTCSCRPGYTGATCELGIDECDPSPCKNGGSCTDLENSYSCTCPPGFYGKICELSAMTCADGPCFNGGRCSDSPDGGYSCRCPVGYSGFNCEKKIDYCSSSPCSNGAKCVDLGDAYLCRCQAGFSGRHCDDNVDDCASSPCANGGTCRDGVNDFSCTCPPGYTGRNCSAPVSRCEHAPCHNGATCHERGHRYVCECARGYGGPNCQFLLPELPPGPAVVDLTEKLEGQGAHHHHHHHHHH
Protein Tag C-His
Accession # O00548
Predicted molecular weight 57.4 kDa
SDS-PAGE 58 kDa, under reducing conditions

Storage and Shipping

Shape Lyophilized
Reconstitution It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store it under sterile conditions at -20℃ to -80℃. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Human DLL1 protein (rp145162) - Protein Bioactivity
Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is typically 2 - 20 μg/mL.

Recombinant Human DLL1 protein (rp145162) - Protein Bioactivity
Measured by its ability to bind human NOTCH1 in a functional ELISA.  Immobilized Recombinant Human DLL1 protein (rp145162) at 2 μg/mL can bind human NOTCH1.

Recombinant Human DLL1 protein (rp145162) - SDS-PAGE
3 μg/lane of Recombinant Human DLL1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 61.1 kDa.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0102358 Certificate of Analysis Feb 04, 2024 rp145162
ZJ24F0102357 Certificate of Analysis Jan 25, 2024 rp145162
ZJ24F0102356 Certificate of Analysis Jan 25, 2024 rp145162

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