Skip to the beginning of the images gallery

Recombinant Human CD4 Protein, >95% (SDS-PAGE), high purity

Grade & Purity:

≥95%(SDS-PAGE)

Expression System: HEK293
Accession #: P01730
Protein Tag: C-His
Bioactivity: Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 40.0 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CD4 protein (rp175911) at 1.0 μg/mL can bind Ibalizumab (anti-CD4) (Ab170728) with the EC50 of 52.66 ng/mL.
Endotoxin Concentration: <1.0 EU/μg

In stock

Item Number

rp175911

Grouped product items
SKU	Size	Availability	Price
rp175911-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$139.90
rp175911-50μg	50μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$259.90
rp175911-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$399.90
rp175911-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,999.90

Animal Free, >95% (SDS-PAGE), Active, 293F cell, His tag, 26-390 aa

View related series

Click to view protein information: CD4

Accession#:P01730 (27) CD4 (36) Gene ID:920 (27)

Basic Description

Product Name	Recombinant Human CD4 Protein, >95% (SDS-PAGE), high purity
Synonyms	CD 4 \| CD4 \| CD4 (L3T4) \| CD4 antigen \| CD4 antigen (p55) \| CD4 molecule \| CD4 receptor \| CD4+ Lymphocyte deficiency, included \| CD4_HUMAN \| CD4mut \| Leu3 \| L3T4 \| Ly-4 \| Lymphocyte antigen CD4 \| MGC165891 \| OTTHUMP00000238897 \| p55 \| T cell antigen T4 \|
Grade	ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description	Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. Mature human CD4 consists of a 371 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain. Within the ECD, human CD4 shares approximately 52% aa sequence identity with mouse and rat CD4. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity. CD4 is reexpressed on circulating CD8+ T cells upon activation and contributes to their cytotoxic effector activity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse. CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1.
Specifications & Purity	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Purity	>95% (SDS-PAGE)
Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 40.0 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CD4 protein (rp175911) at 1.0 μg/mL can bind Ibalizumab (anti-CD4) (Ab170728) with the EC50 of 52.66 ng/mL.
Endotoxin Concentration	<1.0 EU/μg
Expression System	HEK293
Amino Acids	26-390 aa
Sequence	KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWHHHHHH
Protein Tag	C-His
Accession #	P01730
Predicted molecular weight	41.5 kDa
SDS-PAGE	45.9-52.1 kDa, reducing condition; 42.8-49.7 kDa, non-reducing condition

Storage and Shipping

Shape	Lyophilized
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

Protein Bioactivity: Recombinant Human CD4 Protein (rp175911)

Recombinant Human CD4 Protein (rp175911) - Protein Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. The ED₅₀ for this effect is 40.0 ng/mL.

ELISA: Recombinant Human CD4 Protein (rp175911)

Recombinant Human CD4 Protein (rp175911) - ELISA
Measured by its binding ability in a functional ELISA. Immobilized Human CD4 protein (rp175911) at 1.0 μg/mL can bind Ibalizumab (anti-CD4) (Ab170728) with the EC₅₀ of 52.66 ng/mL.

SDS-PAGE: Recombinant Human CD4 Protein (rp175911)

Recombinant Human CD4 Protein (rp175911) - SDS-PAGE
3 μg/lane of Recombinant Human CD4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 45.9-52.1 kDa under reducing conditions and 42.8-49.7 kDa under non-reducing conditions.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

1 results found

Lot Number	Certificate Type	Date	Item
ZJ24R0200294	Certificate of Analysis	Feb 29, 2024	rp175911

Documents & Articles

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration