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Recombinant Human CD31/PECAM-1 Protein

Grade & Purity:

≥95%(SDS-PAGE)
See COA

Accession #: P16284
Bioactivity: Measured by the ability of the immobilized protein to support the adhesion of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.77 μg/mL.
Endotoxin Concentration: <1.0 EU/μg

In stock

Item Number

rp181314

Grouped product items
SKU	Size	Availability	Price
rp181314-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$139.90
rp181314-50μg	50μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$379.90
rp181314-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$599.90
rp181314-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$3,399.90

Animal Free, ≥95% (SDS-PAGE), Active, HEK293, C-His, 1-601 aa

View related series

Click to view protein information: PECAM1

Accession#:P16284 (7) CD31/PECAM-1 (1) Gene ID:5175 (7) PECAM1 (8)

Basic Description

Product Name	Recombinant Human CD31/PECAM-1 Protein
Synonyms	adhesion molecule \| CD31 antigen \| CD31 \| CD31/EndoCAM \| EndoCAM \| FLJ34100 \| FLJ58394 \| GPIIA' \| PECA1 \| PECAM1 \| PECAM-1 \| PECAM-1, CD31/EndoCAM \| platelet endothelial cell adhesion molecule \| platelet endothelial cell adhesion molecule-1 \| platelet/end
Grade	ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance
Product Description	The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes. Post-translational: Phosphorylated on Ser and Tyr residues after cellular activation by src kinases (PubMed:18710921, PubMed:19342684, PubMed:21464369, PubMed:9298995). Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690 (PubMed:21464369). Constitutively phosphorylated on Ser-734 in resting platelets (PubMed:21464369). Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation (PubMed:18710921). Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.
Specifications & Purity	Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, ≥95%(SDS-PAGE), See COA
Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.77 μg/mL.
Endotoxin Concentration	<1.0 EU/μg
Amino Acids	1-601 aa
Sequence	MQPRWAQGATMWLGVLLTLLLCSSLEGQENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQVLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKASKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKHHHHHH
Accession #	P16284
Predicted molecular weight	65.3 kDa
SDS-PAGE	87.7-107.7 kDa, under reducing conditions; 82.4-102.5 kDa, under non-reducing conditions.

Storage and Shipping

Concentration	See COA
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C for 1 year. Avoid freeze/thaw cycle.

Images

Bioactivity: Recombinant Human CD31/PECAM-1 Protein (rp181314)

Recombinant Human CD31/PECAM-1 Protein (rp181314) - Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.77 μg/mL.

SDS-PAGE: Recombinant Human CD31/PECAM-1 Protein (rp181314)

Recombinant Human CD31/PECAM-1 Protein (rp181314) - SDS-PAGE
3 μg/lane of Recombinant Human CD31/PECAM-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 87.7-107.7 kDa under reducing conditions and 82.4-102.5 kDa under non-reducing conditions.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

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