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Recombinant Human CD300c Protein, ≥95% (SDS-PAGE), high purity

  • Expression System: HEK293
  • Accession #: Q08708
  • Protein Tag: C-His
  • Bioactivity: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL antiCD3 and Recombinant Human CD300c Fc Chimera. The ED50 for this effect is 4-16 μg/mL.
  • Endotoxin Concentration: <0.1 EU/μg
In stock
Item Number
rp143943
Grouped product items
SKU Size
Availability
Price Qty
rp143943-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90
rp143943-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
rp143943-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$499.90
rp143943-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F, C-His tag, 21-183 aa

Basic Description

Product Name Recombinant Human CD300c Protein, ≥95% (SDS-PAGE), high purity
Synonyms CD300 antigen-like family member C | CLM-6 | CMRF-35 | CMRF35-A1 | IgSF16 | Immunoglobulin superfamily member 16 | CD300 antigen-like family member C | CD300c | CD300c antigen | CD300c molecule | CLM-6 | CLM6_HUMAN | CMRF-35 | CMRF-35A | CMRF35 | CMRF35 a
Grade ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity
≥95% (SDS-PAGE)
Endotoxin level
<0.1 EU/μg
Function
Leukocyte mono-Ig-like receptor 2 (LMIR2; also CMRF-35, CMRF35-A antigen, and CD300c antigen) is a 23 kDa (predicted) type I transmembrane glycoprotein that belongs to the immunoregulatory signaling (IRS) family of molecules within the immunoglobulin (Ig) superfamily. Human LMIR2 is synthesized as a 224 amino acid (aa) precursor that has a 20 aa signal sequence, a 163 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 20 aa cytoplasmic tail (SwissProt # Q08708). The ECD contains an Ig-like V-type domain (aa’s 22-128) and two N-linked glycosylation sites (aa’s 90 and 99). Downstream of the Ig V-domain, the membrane proximal region of LMIR2 (aa 128-183) contains a high proportion of proline (18%), serine (20%) and threonine (13%) residues. The transmembrane segment contains a charged glutamic acid that contributes to cell activation. Human LMIR2 shares 52% aa sequence identity with the mouse LMIR2, which is also known as CLM4. Human LMIR2 is 90% identical to human LMIR1 within the Ig-like domain. LMIR2 is present on the surface of natural killer cells, granulocytes, most myeloid cells, dendritic cells, and a subpopulation of T and B lymphocytes.

Specifications & Purity ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Bioactivity Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL antiCD3 and Recombinant Human CD300c Fc Chimera. The ED50 for this effect is 4-16 μg/mL.
Endotoxin Concentration <0.1 EU/μg
Expression System HEK293
Species Human
Amino Acids 21-183 aa
Sequence GYFPLSHPMTVAGPVGGSLSVQCRYEKEHRTLNKFWCRPPQILRCDKIVETKGSAGKRNGRVSIRDSPANLSFTVTLENLTEEDAGTYWCGVDTPWLRDFHDPIVEVEVSVFPAGTTTASSPQSSMGTSGPPTKLPVHTWPSVTRKDSPEPSPHPGSLFSNVRHHHHHH
Protein Tag C-His
Accession # Q08708
Predicted molecular weight 45 kDa
SDS-PAGE 40 - 50 kDa, under reducing conditions; 40 - 50 kDa, under non-reducing conditions.

Storage and Shipping

Shape Lyophilized
Reconstitution Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20~-80℃ for more than 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Human CD300c Protein (rp143943) - SDS-PAGE
3 μg/lane of Recombinant Human CD300c Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 40 - 50 kDa.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

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