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Recombinant Human CCL23/MPIF-1 Protein, ＞97%(SDS-PAGE and HPLC), high purity

Grade & Purity:

≥97%(SDS-PAGE&HPLC)

Expression System: E.coli
Accession #: P55773
Protein Tag: No tag
Bioactivity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/ml.
Endotoxin Concentration: <1.0 EU/μg

In stock

Item Number

rp143739

Grouped product items
SKU	Size	Availability	Price
rp143739-10μg	10μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$69.90
rp143739-50μg	50μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$299.90
rp143739-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$499.90
rp143739-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,999.90

Animal Free, >97%(SDS-PAGE and HPLC), Active, E.coli, No tag, 22-120 aa

View related series

Click to view protein information: CCL23

Accession#:P55773 (3) CCL23 (3) Gene ID:6368 (3) MPIF-1 (2)

Basic Description

Product Name	Recombinant Human CCL23/MPIF-1 Protein, ＞97%(SDS-PAGE and HPLC), high purity
Synonyms	C C motif chemokine ligand 23 \| C-C motif chemokine 23 \| C6 beta chemokine \| CCL 23 \| CCL23 \| CCL23(30-99) \| CCL23_HUMAN \| Chemokine (C C motif) ligand 23 \| Chemokine CC motif ligand 23 \| ck beta 8 \| CK-beta-8 \| CKB 8 \| CKB-8 \| Ckb-8-1 \| CKB8 \| Hmrp-2a \|
Grade	ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description	Purity >97% by SDS-PAGE and HPLC analyses. Function Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. Myeloid progenitor inhibitory factor (MPIF-1), also known as CK beta 8 and MIP-3, is a member of the CC chemokine subfamily that is designated CCL23. Alternative splicing of the MPIF-1 gene results in two mRNAs that encode a short (CK beta 8) and a long (CK beta 8-1) isoform of the chemokine. CK beta 8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CK beta 8 (aa 22 - 120). Additional N-terminal processing of the 99 aa residue variant can generate a 75 aa residue CK beta 8 (aa 46 - 120) that is significantly more active than the 99 aa residue variant. Similarly, CK beta 8-1 encodes a 137 aa residue precursor protein that can give rise to a 116 and a 92 aa residue chemokine. Among CC chemokine members, MPIF-1 is most closely related to MIP-5/CCL15 (67% sequence identity) and MIP-1 alpha /CCL3 (51%). MPIF-1 mRNA is most abundant in the adult lung and liver, but is also present in bone marrow, placenta, and various myelomonocytic cell lines. MPIF-1 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages. MPIF-1 binds to CCR1 with high affinity and has been shown to be a potent chemoattractant and activator of monocytes, dendritic cells, and osteoclast precursors.
Specifications & Purity	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC)
Purity	＞97%(SDS-PAGE and HPLC)
Bioactivity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/ml.
Endotoxin Concentration	<1.0 EU/μg
Expression System	E.coli
Species	Human
Amino Acids	22-120 aa
Sequence	RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN
Protein Tag	No tag
Protein Length	Full length protein
Accession #	P55773
Source	Recombinant
Predicted molecular weight	11.4 kDa
SDS-PAGE	11.4 kDa

Storage and Shipping

Shape	Lyophilized
Reconstitution	Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst

Images

Protein Bioactivity: Recombinant Human CCL23/MPIF-1 Protein (rp143739)

Recombinant Human CCL23/MPIF-1 Protein (rp143739)-Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/mL.

SDS-PAGE: Recombinant Human CCL23/MPIF-1 Protein (rp143739)

Recombinant Human CCL23/MPIF-1 Protein (rp143739)-SDS-PAGE
Recombinant Human CCL23/MPIF-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 10.4 kDa under reducing conditions and 16.0-18.5 kDa under non-reducing conditions.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

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Analytical Chart:

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3 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0600374	Certificate of Analysis	Mar 26, 2024	rp143739
ZJ23F0600373	Certificate of Analysis	Mar 26, 2024	rp143739
ZJ23F0600375	Certificate of Analysis	Mar 26, 2024	rp143739

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