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Recombinant Human 5T4/TPBG Protein

COA

Grade & Purity:

Animal Free

Carrier Free

≥95%(SDS-PAGE)

Expression System: HEK293
Accession #: Q13641
Protein Tag: C-His
Bioactivity: Testing in progress
Endotoxin Concentration: <1.0 EU/μg

In stock

Item Number

rp183650

Grouped product items
SKU	Size	Availability	Price
rp183650-10μg	10μg	≥10	$59.90
rp183650-50μg	50μg	10	$139.90
rp183650-100μg	100μg	10	$219.90
rp183650-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,599.90

Animal Free, ≥95% (SDS-PAGE), 293F cell, C-His tag, 1-355 aa

View related series

Click to view protein information: TPBG

5T4/TPBG (1) Accession#:Q13641 (4) Gene ID:7162 (4) TPBG (4)

Basic Description

Product Name	Recombinant Human 5T4/TPBG Protein
Synonyms	5T4 oncotrophoblast glycoprotein \| 5T4 \| 5T4AG \| 5T4-AG \| 5T4M6P1,5T4 oncofetal antigen \| M6P1 \| TPBG \| trophoblast glycoprotein \| 5T4 oncofetal trophoblast glycoprotein
Grade	Animal Free, Carrier Free
Product Description	5T4, also known as Trophoblast glycoprotein and Wnt-activated inhibitory factor 1 (WAIF1), is a single-pass transmembrane protein which may function as an inhibitor of Wnt/beta-catenin signaling. Distinct regions on the 5T4 ectodomain which contains eight leucine-rich repeats are thought to antagonize Wnt signaling. 5T4 has limited expression in normal adult tissues and in some solid tumors, while expression is elevated in human carcinomas which have been correlated with poor survival and prognosis. Human 5T4 is a 420 amino acid (aa) protein that includes a 31 aa signal peptide, a 324 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD, human 5T4 shares 78% and 77% aa sequence identity with mouse and rat 5T4, respectively. It has been identified that TIP-2/GIPC, a common interacting protein involved in cancer, interacts with 5T4 through its PDZ domain. 5T4 inhibits Wnt/ beta -catenin signaling by binding to LRP6 and inhibit Wnt-induced LRP6 internalization into endocytic vesicles. Conversely, 5T4 can activate noncanonical Wnt signaling through DKK1. In addition to LRP6, in-house data indicates 5T4 can also interact with Frizzled-8.
Specifications & Purity	Animal Free, Carrier Free, ≥95%(SDS-PAGE)
Bioactivity	Testing in progress
Endotoxin Concentration	<1.0 EU/μg
Expression System	HEK293
Amino Acids	1-355 aa
Sequence	MPGGCSRGPAAGDGRLRLARLALVLLGWVSSSSPTSSASSFSSSAPFLASAVSAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPAYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRAGAFEHLPSLRQLDLSHNPLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTSHHHHHH
Protein Tag	C-His
Accession #	Q13641
Predicted molecular weight	36.1 kDa
SDS-PAGE	47.9-66.8 kDa, under reducing conditions; 47.9-66.8 kDa, under non-reducing conditions

Storage and Shipping

Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

SDS-PAGE: Recombinant Human 5T4/TPBG Protein (rp183650)

Recombinant Human 5T4/TPBG Protein (rp183650) - SDS-PAGE
2 μg/lane of Recombinant Human 5T4/TPBG Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 47.9-66.8 kDa.

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Analytical Chart:

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4 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0708010	Certificate of Analysis	Jul 12, 2024	rp183650
ZJ24F0708008	Certificate of Analysis	Jul 12, 2024	rp183650
ZJ24F0708009	Certificate of Analysis	Jul 12, 2024	rp183650
ZJ24R0600686	Certificate of Analysis	Jun 14, 2024	rp183650

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