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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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rp214462-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$89.90
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rp214462-50μg
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50μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$269.90
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rp214462-100μg
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100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$429.90
|
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rp214462-1mg
|
1mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$3,399.90
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Carrier Free, ≥90% (SDS-PAGE), E. coli, N-His, 2-246 aa
| Product Name | Recombinant Human 14-3-3 beta/alpha Protein |
|---|---|
| Synonyms | 14-3-3 alpha | 14-3-3 protein beta/alpha | 1433B_HUMAN | Brain protein 14-3-3 beta isoform | GW128 | HS 1 | KCIP-1 | KCIP1 | N-terminally processed | Protein 1054 | Protein kinase C inhibitor protein 1 | YWHAA | YWHAB |
| Grade | Carrier Free |
| Specifications & Purity | Carrier Free, ≥90%(SDS-PAGE), See COA |
| Biochemical and Physiological Mechanisms | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the |
| Endotoxin Concentration | <1.0 EU/μg |
| Amino Acids | 2-246 aa |
| Sequence | MHHHHHHTMDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVLELLDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLNEESYKDSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN |
| Accession # | P31946 |
| Predicted molecular weight | 28.9 kDa |
| SDS-PAGE | 27.9 kDa, under reducing conditions; 27.5 & 28.1 & 54.3 kDa, under non-reducing conditions. |
| Concentration | See COA |
|---|---|
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20℃ for 12 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
Recombinant Human 14-3-3 beta/alpha Protein (rp214462) - SDS-PAGE
2 μg/lane of Recombinant Human 14-3-3 beta/alpha Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 27.9 kDa under reducing conditions and 27.5 & 28.1 & 54.3 kDa under non-reducing conditions.
14-3-3 beta/alpha can form homodimer (Uniptot: P31946).
Under non-reducing conditions, the cysteine residues of YWHAB form different disulfide bonds (Cys12-Cys214 or Cys26-Cys189), leading to structural domain rearrangements that enable the protein to adopt two distinct conformations (PMID: 15347690).