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Recombinant HLA-DR Antibody (PE-Cy7) - Primary antibody, specific to Human HLA-DR

    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2a
  • Clone Number: P22B24
  • Conjugation: PE-Cy7
In stock
Item Number
Ab223651
Grouped product items
SKU Size
Availability
Price Qty
Ab223651-10T
10T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$72.90
Ab223651-25T
25T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$144.90
Ab223651-100T
100T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$294.90

Recombinant (P22B24); Mouse anti Human HLA-DR Antibody (PE-Cy7); Flow Cytometry

Basic Description

Product Name Recombinant HLA-DR Antibody (PE-Cy7) - Primary antibody, specific to Human HLA-DR
Specifications & Purity Recombinant, ExactAb™, Validated, 5 μL/test
Biochemical and Physiological Mechanisms An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell recep
Host species Mouse
Specificity Human HLA-DR
Immunogen Human lymphoblastoid B-cell line RPMI 8866
Positive Control Flow Cytometry: Human peripheral blood lymphocytes; Raji cells
Conjugation PE-Cy7
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 774nm
Ex/Em(nm) Ex:565nm;Em:774nm
Grade ExactAb™, Recombinant, Validated
Product Description

Recombinant HLA-DR Antibody, clone P22B24, could be used for Flow Cytometry.


Product Properties

Light Chain Type kappa
Purification Method Protein A purified
Concentration 5 μL/test
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

HLA-DRA Tbio HLA class II histocompatibility antigen, DR alpha chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant HLA-DR Antibody (PE-Cy7) (Ab223651) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant HLA-DR Antibody (PE-Cy7) (Ab223651) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE-Cy7) (Ab190079).

Recombinant HLA-DR Antibody (PE-Cy7) (Ab223651) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant HLA-DR Antibody (PE-Cy7) (Ab223651) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy7) (Ab190079). Black - Unlabelled control, cells without incubation with primary antibody.

Recombinant HLA-DR Antibody (PE-Cy7) (Ab223651) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by Raji cells (red) compared to an isotype control (blue). Cells were stained with Recombinant HLA-DR Antibody (PE-Cy7) (Ab223651) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy7) (Ab190079). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

5.0 μL/test.
For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with Mouse FcR blocking reagent as 5.0 μL per 106 cells in 100 µl volume for 30 minutes at 4℃ prior to immunostai

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

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