Skip to the beginning of the images gallery

Recombinant HLA-DR Antibody (AF405) - Primary antibody, specific to Human HLA-DR

Grade & Purity:

5 μL/test

Application:

Flow Cytometry

Host species: Mouse
Reactive species: Human
Isotype: Mouse IgG2a
Clone Number: P22B24
Conjugation: AF405

In stock

Item Number

Ab223628

Grouped product items
SKU	Size	Availability	Price
Ab223628-10T	10T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$49.90
Ab223628-25T	25T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$94.90
Ab223628-100T	100T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$227.90

Recombinant (P22B24); Mouse anti Human HLA-DR Antibody (AF405); Flow Cytometry

View related series

Click to view target information: HLA-DRA

Accession#:P01903 (22) AF405 (73) Gene ID:3122 (22) HLA-DR (18) HLA-DRA (22)

Basic Description

Product Name	Recombinant HLA-DR Antibody (AF405) - Primary antibody, specific to Human HLA-DR
Specifications & Purity	Recombinant, ExactAb™, Validated, 5 μL/test
Biochemical and Physiological Mechanisms	An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell recep
Host species	Mouse
Specificity	Human HLA-DR
Immunogen	Human lymphoblastoid B-cell line RPMI 8866
Positive Control	Flow Cytometry: Human peripheral blood lymphocytes; Raji cells
Conjugation	AF405
Excitation(nm)	Violet Laser(405nm)
Emission(nm)	422nm
Ex/Em(nm)	Ex:401nm;Em:422nm
Grade	ExactAb™, Recombinant, Validated
Product Description	Recombinant HLA-DR Antibody, clone P22B24, could be used for Flow Cytometry.

Product Properties

Light Chain Type	kappa
Purification Method	Protein A purified
Concentration	5 μL/test
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

HLA-DRA Tbio HLA class II histocompatibility antigen, DR alpha chain (0 Activities)

Discover Target: HLA-DRA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Flow Cytometry: Recombinant HLA-DR Antibody (AF405) (Ab223628)

Recombinant HLA-DR Antibody (AF405) (Ab223628) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant HLA-DR Antibody (AF405) (Ab223628) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF405) (Ab190076).

Flow Cytometry: Recombinant HLA-DR Antibody (AF405) (Ab223628)

Recombinant HLA-DR Antibody (AF405) (Ab223628) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant HLA-DR Antibody (AF405) (Ab223628) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Recombinant HLA-DR Antibody (AF405) (Ab223628)

Recombinant HLA-DR Antibody (AF405) (Ab223628) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by Raji cells (red) compared to an isotype control (blue). Cells were stained with Recombinant HLA-DR Antibody (AF405) (Ab223628) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Recombinant HLA-DR Antibody (AF405) - Primary antibody, specific to Human HLA-DR

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews