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Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (AF647) - Primary antibody, specific to DYKDDDDK epitope tag

Grade & Purity:

0.5 mg/mL

Application:

Flow Cytometry
IF/ICC

Reactive species: All species
Isotype: Mouse IgG2a
Clone Number: P06A32
Conjugation: AF647

In stock

Item Number

Ab218452

Grouped product items
SKU	Size	Availability	Price
Ab218452-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$49.90
Ab218452-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$129.90
Ab218452-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$199.90
Ab218452-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,299.90

Recombinant (P06A32); Mouse anti Flag tag Antibody (AF647); Flow Cytometry; IF/ICC

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AF647 (119) DYKDDDDK (13) Flag (13)

Basic Description

Product Name	Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (AF647) - Primary antibody, specific to DYKDDDDK epitope tag
Specifications & Purity	ExactAb™, Validated, Recombinant, High Performance, 0.5 mg/mL
Biochemical and Physiological Mechanisms	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK ta
Specificity	DYKDDDDK epitope tag
Immunogen	Synthetic DYKDDDDK peptide
Positive Control	IF/ICC: HEK293 cells transfected with Flag and eGFP tags Flow Cytometry: HEK293 cells transfected with Flag and eGFP tags
Conjugation	AF647
Excitation(nm)	Red Laser(633nm)
Emission(nm)	668nm
Ex/Em(nm)	Ex:650nm;Em:668nm
Grade	ExactAb™, High Performance, Recombinant, Validated
Product Description	Recombinant DYKDDDDK Antibody, clone P06A32, could be used for Flow Cytometry, IF/ICC and so on.

Product Properties

Light Chain Type	kappa
Purification Method	Protein A purified
Concentration	0.5 mg/mL
Storage Temp	Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Images

IF/ICC: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (AF647) (Ab218452)

Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (AF647) (Ab218452) - IF/ICC
IF analysis of DYKDDDDK tag (Binds to Flag tag) (Red) in HEK293T cells transfected with a EGFP-DYKDDDDK tag. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (AF647) (Ab218452) at 1/1000 dilution in blocking buffer for 2 hours at RT in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (AF647) (Ab218452)

Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (AF647) (Ab218452) - Flow Cytometry
Flow Cytometry analysis of DYKDDDDK tag (Binds to Flag tag) expression by HEK293T cells transfected with a EGFP-DYKDDDDK tag (Right panel) compared to an isotype control (Left panel). The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes and then stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (AF647) (Ab218452) at a dilution of 1/300 for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (AF647) (Ab190077).

Flow Cytometry: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (AF647) (Ab218452)

Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (AF647) (Ab218452) - Flow Cytometry
Flow Cytometry analysis of DYKDDDDK tag (Binds to Flag tag) expression by HEK293T cells transfected with a EGFP-DYKDDDDK tag (red) compared to an isotype control (blue). The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes and then stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (AF647) (Ab218452) at a dilution of 1/300 for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF647) (Ab190077).

Application

Application	Dilution info
Flow Cytometry	1/100-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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