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Recombinant CD8α Antibody (Biotin) - Primary antibody, specific to Human CD8 alpha chain

Grade & Purity:

0.5 mg/mL

Application:

Flow Cytometry

Host species: Mouse
Reactive species: Human
Isotype: Mouse IgG1
Clone Number: P20A30
Conjugation: Biotin

In stock

Item Number

Ab223585

Grouped product items
SKU	Size	Availability	Price
Ab223585-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$16.90
Ab223585-25μg	25μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$31.90
Ab223585-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$99.90

Recombinant (P20A30); Mouse anti Human CD8α Antibody (Biotin); Flow Cytometry

View related series

Click to view target information: CD8A

Accession#:P01732 (42) CD8A (53) Gene ID:925 (42) MAL (22)

Basic Description

Product Name	Recombinant CD8α Antibody (Biotin) - Primary antibody, specific to Human CD8 alpha chain
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.5 mg/mL
Biochemical and Physiological Mechanisms	Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide com
Host species	Mouse
Specificity	Human CD8 alpha chain
Immunogen	Human CD8a
Positive Control	Flow Cytometry: Human peripheral blood lymphocytes
Conjugation	Biotin
Grade	ExactAb™, Recombinant, Validated
Product Description	Recombinant CD8α Antibody, clone P20A30, could be used for Flow Cytometry.

Product Properties

Light Chain Type	kappa
Purification Method	Protein A purified
Concentration	0.5 mg/mL
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Flow Cytometry: Recombinant CD8α Antibody (Biotin) (Ab223585)

Recombinant CD8α Antibody (Biotin) (Ab223585) - Flow Cytometry
Flow cytometry analysis of CD8α expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD8α Antibody (Biotin) (Ab223585) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

Flow Cytometry: Recombinant CD8α Antibody (Biotin) (Ab223585)

Recombinant CD8α Antibody (Biotin) (Ab223585) - Flow Cytometry
Flow cytometry analysis of CD8α expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD8α Antibody (Biotin) (Ab223585) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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