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SKU | Size | Availability |
Price | Qty |
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Ab213870-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$15.90
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Ab213870-25μg
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25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$29.90
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Ab213870-100μg
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100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$83.90
|
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Recombinant (P83E51); Rat anti Mouse CD8α Antibody (AF647); Flow Cytometry
Product Name | Recombinant CD8 alpha Antibody (AF647) - Primary antibody, specific to Mouse CD8α |
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Synonyms | alpha polypeptide (p32) antibody | CD_antigen=CD8a antibody | CD8 antibody | CD8 antigen alpha polypeptide antibody | CD8 antigen alpha polypeptide (p32) antibody | CD8 antigen, alpha polypeptide (p32) antibody | CD8a antibody | CD8A antigen antibody | CD |
Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
Biochemical and Physiological Mechanisms | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide com |
Host species | Rat |
Specificity | Mouse CD8α |
Immunogen | Mouse Spleen Cells or Thymocyte Membranes |
Positive Control | Flow Cytometry: C57BL/6 mouse splenocytes |
Conjugation | AF647 |
Excitation(nm) | Red Laser(633nm) |
Emission(nm) | 668nm |
Ex/Em(nm) | Ex:650nm;Em:668nm |
Grade | ExactAb™, Validated |
Product Description |
Recombinant CD8α Antibody, clone P83E51, could be used for Flow Cytometry. |
Light Chain Type | kappa |
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SDS-PAGE | 150 kDa |
Purification Method | Protein A purified |
Concentration | 0.5 mg/mL |
Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
Shipped In | Wet ice |
Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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Recombinant CD8 alpha Antibody (AF647) (Ab213870) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD8 alpha Antibody (AF647) (Ab213870) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF647) (Ab190071).
Recombinant CD8 alpha Antibody (AF647) (Ab213870) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD8 alpha Antibody (AF647) (Ab213870) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.
Application | Dilution info |
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Flow Cytometry | ≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume. |