Skip to the beginning of the images gallery

Recombinant CD14 Antibody (AF750) - Primary antibody, specific to Human CD14

COA

Grade & Purity:

Validated

ExactAb™

5 μL/test

Application:

Flow Cytometry

Host species: Mouse
Reactive species: Human
Isotype: Mouse IgG2a
Clone Number: P02C06
Conjugation: AF750

In stock

Item Number

Ab223475

Grouped product items
SKU	Size	Availability	Price
Ab223475-10T	10T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$59.90
Ab223475-25T	25T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$119.90
Ab223475-100T	100T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$189.90

Recombinant (P02C06); Mouse anti Human CD14 Antibody (AF750); Flow Cytometry

View related series

Click to view target information: CD14

Accession#:P08571 (46) AF750 (22) CD14 (50) Gene ID:929 (46)

Basic Description

Product Name	Recombinant CD14 Antibody (AF750) - Primary antibody, specific to Human CD14
Specifications & Purity	ExactAb™, Validated, 5 μL/test
Biochemical and Physiological Mechanisms	Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and
Host species	Mouse
Specificity	Human CD14
Immunogen	Human Thymocytes followed by peripheral blood mononuclear cells.
Positive Control	Flow Cytometry: Human peripheral blood monocytes
Conjugation	AF750
Excitation(nm)	Red Laser(633nm)
Emission(nm)	776nm
Ex/Em(nm)	Ex:752nm;Em:776nm
Grade	ExactAb™, Validated
Product Description	Recombinant CD14 Antibody, clone P02C06, could be used for Flow Cytometry.

Product Properties

Light Chain Type	kappa
Purification Method	Protein A purified
Concentration	5 μL/test
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD14 Tbio Monocyte differentiation antigen CD14 (0 Activities)

Discover Target: CD14

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Flow Cytometry: Recombinant CD14 Antibody (AF750) (Ab223475)

Recombinant CD14 Antibody (AF750) (Ab223475) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD14 Antibody (AF750) (Ab223475) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF750) (Ab190249).

Flow Cytometry: Recombinant CD14 Antibody (AF750) (Ab223475)

Recombinant CD14 Antibody (AF750) (Ab223475) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD14 Antibody (AF750) (Ab223475) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF750) (Ab190249). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration