Grouped product items
SKU	Size	Availability	Price
Ab210601-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$23.90
Ab210601-50μg	50μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab210601-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$112.90
Ab210601-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$599.90

Recombinant (5B2); Mouse anti BrdU Antibody; ICC/IF, ELISA; Unconjugated

View related series

BrdU (5)

Basic Description

Product Name	Recombinant BrdU Antibody - Primary antibody, specific to BrdU
Synonyms	Bromodeoxyuridine antibody \| BrdU antibody \| Anti-BrdU antibody \| 5-bromo-2-deoxyuridine antibody \| BrdU Monoclonal Antibody \| Anti-BrdU/5-BrdU Antibody \| Anti BrdU
Specifications & Purity	Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), See CO
Biochemical and Physiological Mechanisms	The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent
Host species	Mouse
Specificity	BrdU
Immunogen	Bromodeoxyuridine
Positive Control	ICC/IF: HeLa cells
Conjugation	Unconjugated
Grade	Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated
Product Description	Recombinant BrdU Antibody, clone 5B2, could be used for ICC/IF, ELISA and so on.

Product Properties

Light Chain Type	lambda
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Concentration	See CO
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

IF/ICC: Recombinant BrdU Antibody (Ab210601)

Recombinant BrdU Antibody (Ab210601) - IF/ICC
IF analysis of Recombinant BrdU Antibody (Green) in HeLa cells. The cells were fixed with ethanol for 15 minutes and subjected to acid hydrolysis using 2M HCl for 15 minutes at room temperature to denature the DNA. They were then stained Recombinant BrdU Antibody (Ab210601) at 1.0 μg/mL in blocking buffer overnight at 4℃ and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application	Dilution info
IF/ICC	1.0 μg/mL
IF/ICC	1.0 μg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Recombinant BrdU Antibody - Primary antibody, specific to BrdU