Grouped product items
SKU	Size	Availability	Price
Ab221524-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$59.90
Ab221524-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$159.90
Ab221524-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$239.90
Ab221524-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,499.90

Recombinant (P88A24); Mouse anti Human beta Tubulin Antibody; WB, Flow Cytometry, IF/ICC, ELISA; Unconjugated

View related series

Click to view target information: TUBB

Accession#:P07437 (8) beta Tubulin (7) Gene ID:203068 (8) TUBB (7)

Basic Description

Product Name	Recombinant beta Tubulin Antibody - Primary antibody, specific to Human beta tubulin
Synonyms	Beta 4 tubulin antibody \| Beta 5 tubulin antibody \| beta Ib tubulin antibody \| Beta1 tubulin antibody \| Class I beta tubulin antibody \| M40 antibody \| MGC117247 antibody \| MGC16435 antibody \| OK/SW cl.56 antibody \| OK/SWcl.56 antibody \| TBB5_HUMAN antibod
Specifications & Purity	Animal Free, Carrier Free, Endotoxin Free, ExactAb™, Validated, High Performance, Recombinant, ≥95%(SDS-PAGE), 0.5 mg/mL
Biochemical and Physiological Mechanisms	Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a
Host species	Mouse
Specificity	Human beta tubulin
Immunogen	Recombinant human beta tubulin
Positive Control	WB: HEK293, HeLa, MCF-7, A431, NIH3T3 and C6 whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells
Conjugation	Unconjugated
Grade	Animal Free, Carrier Free, Endotoxin Free, ExactAb™, High Performance, Recombinant, Validated
Product Description	Recombinant beta Tubulin Antibody, clone P88A24, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Product Properties

Light Chain Type	kappa
SDS-PAGE	150.0 KDa
Purification Method	Protein A purified
Concentration	0.5 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

TUBB Tclin Tubulin beta chain (0 Activities)

Discover Target: TUBB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: Recombinant beta Tubulin Antibody (Ab221524)

Recombinant beta Tubulin Antibody (Ab221524) - Western Blot
All lanes: Recombinant beta Tubulin Antibody (Ab221524) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 51 kDa
Exposure time: 11.9 s

Flow Cytometry: Recombinant beta Tubulin Antibody (Ab221524)

Recombinant beta Tubulin Antibody (Ab221524) - Flow Cytometry 
Flow Cytometry analysis of HeLa cells stained with Recombinant beta Tubulin Antibody (Ab221524) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (FITC) (Ab179003) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2a-kappa, anti-A29L (Ab210599) was used as an isotype control (Blue).

IF/ICC: Recombinant beta Tubulin Antibody (Ab221524)

Recombinant beta Tubulin Antibody (Ab221524) - IF/ICC
IF analysis of beta Tubulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant beta Tubulin Antibody (Ab221524) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application	Dilution info
WB	1/500-1/10000
Flow Cytometry	1/200 - 1/12500
IF/ICC	1/250

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

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Analytical Chart:

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ25F0622992	Certificate of Analysis	Jun 23, 2025	Ab221524
ZJ25F0622991	Certificate of Analysis	Jun 23, 2025	Ab221524

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Recombinant beta Tubulin Antibody - Primary antibody, specific to Human beta tubulin