Skip to the beginning of the images gallery

Recombinant 14-3-3 alpha/beta/zeta/delta Antibody - Primary antibody, specific to 14-3-3 alpha/beta/zeta/delta

Grade & Purity:

See COA

Application:

Host species: Rabbit
Reactive species: Human,Mouse,Rat
Isotype: Rabbit IgG
Conjugation: Unconjugated

In stock

Item Number

Ab086582

Grouped product items
SKU	Size	Availability	Price
Ab086582-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab086582-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$199.90
Ab086582-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab086582-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,299.90

Recombinant; Rabbit anti Human 14-3-3 alpha/beta/zeta/delta Antibody; WB, IHC; Unconjugated

View related series

Click to view target information: YWHAB Click to view target information: YWHAZ

14-3-3 alpha (2) Accession#:P31946 (3) Accession#:P63104 (7) beta (3) delta (2) Gene ID:7529 (3) Gene ID:7534 (7) YWHAB (3) YWHAZ (7) zeta (1)

Basic Description

Product Name	Recombinant 14-3-3 alpha/beta/zeta/delta Antibody - Primary antibody, specific to 14-3-3 alpha/beta/zeta/delta
Synonyms	14 3 3 alpha antibody \| 14 3 3 protein beta/alpha antibody \| 14-3-3 protein beta/alpha antibody \| 1433B_HUMAN antibody \| Brain protein 14 3 3 beta isoform antibody \| GW128 antibody \| HS 1 antibody \| KCIP-1 antibody \| KCIP1 antibody \| N-terminally processe
Specifications & Purity	ExactAb™, Validated, Recombinant, See COA
Biochemical and Physiological Mechanisms	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the
Host species	Rabbit
Specificity	14-3-3 alpha/beta/zeta/delta
Immunogen	A synthetic peptide derived from human 14-3-3 alpha/beta (AA 1-50).
Positive Control	WB: HeLa, Jurkat, Hep G2, MCF7, A549, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Note	可替代货号Ab086584
Product Description	Rabbit anti Human 14-3-3 alpha/beta/zeta/delta Antibody, Recombinant, could be used for WB, IHC and so on.

Product Properties

Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

YWHAB Tbio 14-3-3 protein beta/alpha (0 Activities)

Discover Target: YWHAB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

YWHAZ Tchem 14-3-3 protein zeta/delta (0 Activities)

Discover Target: YWHAZ

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: Recombinant 14-3-3 alpha/beta/zeta/delta Antibody (Ab086582)

Recombinant 14-3-3 alpha/beta/zeta/delta Antibody (Ab086582) - Western Blot
All lanes: Recombinant 14-3-3 alpha/beta/zeta/delta Antibody (Ab086582) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 28, 29 kDa
Exposure time: 9.4 s

IHC: Recombinant 14-3-3 alpha/beta/zeta/delta Antibody (Ab086582)

Recombinant 14-3-3 alpha/beta/zeta/delta Antibody (Ab086582) - IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Recombinant 14-3-3 alpha/beta/zeta/delta Antibody (Ab086582).

Application

Application	Dilution info
WB	1/1000 - 1/2000
IHC	1/100-1/200

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration