Basic Description

Synonyms	EX-A6693 \| FS-5016 \| SCHEMBL4317 \| Adenosine, 3'-(((2S)-2-amino-3-(4-methoxyphenyl)-1-oxopropyl)amino)-3'-deoxy-N,N-dimethyl-, dihydrochloride \| Puromycin HCl \| Tox21_111352 \| P-540 \| Q7320232 \| (S)-3'-((2-Amino-3-(4-methoxyphenyl)-1-oxopropyl)amino)-3'-d
Specifications & Purity	BioReagent, for cell culture
Biochemical and Physiological Mechanisms	Puromycin associates in a non-specific manner with growing polypeptide chains, and thus, leads to premature termination of translation. It inhibits protein synthesis in two ways. It associates with the donor substrate, peptidyl-tRNA, in the P site and thu
Storage Temp	Protected from light,Store at -20°C
Shipped In	Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available.
Grade	BioReagent, for cell culture
Note	注意: As supplied, this product remains active for four years when stored at -20°C.
Product Description	General Description Puromycin is a broad spectrum antibiotic produced by Streptomyces alboniger . It is an analog of the 3′ end of aminoacyl-tRNA. It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes. Chemical structure: peptidyl nucleoside Application Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger . It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL. Puromycin dihydrochloride from Streptomyces alboniger has been used: in the preparation of puromycin stock solution for puromycin sensitivity assay. to select lentivirus-infected cells containing puromycin resistant selection marker. Used as a selective agent for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). Recommended for use at a range of 1-10 μg/ml. Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C. Other Notes Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Nucleosides, nucleotides, and analogues
  - Class Purine nucleosides
    - Subclass Purine 3'-deoxyribonucleosides

Kingdom	Organic compounds
Superclass	Nucleosides, nucleotides, and analogues
Class	Purine nucleosides
Subclass	Purine 3'-deoxyribonucleosides
Intermediate Tree Nodes	Not available
Direct Parent	Purine 3'-deoxyribonucleosides
Alternative Parents	Phenylalanine and derivatives Alpha amino acid amides Glycosylamines 6-alkylaminopurines Amphetamines and derivatives Pentoses Dialkylarylamines Anisoles Methoxybenzenes Phenoxy compounds Aralkylamines Aminopyrimidines and derivatives Alkyl aryl ethers N-substituted imidazoles Imidolactams Fatty amides Tetrahydrofurans Heteroaromatic compounds Secondary carboxylic acid amides Secondary alcohols Oxacyclic compounds Azacyclic compounds Hydrocarbon derivatives Hydrochlorides Monoalkylamines Organic oxides Organopnictogen compounds Primary alcohols Carbonyl compounds
Molecular Framework	Aromatic heteropolycyclic compounds
Substituents	Purine 3'-deoxyribonucleoside - Phenylalanine or derivatives - Alpha-amino acid amide - Glycosyl compound - N-glycosyl compound - 6-alkylaminopurine - 6-aminopurine - Alpha-amino acid or derivatives - Amphetamine or derivatives - Pentose monosaccharide - Imidazopyrimidine - Purine - Phenoxy compound - Anisole - Phenol ether - Methoxybenzene - Dialkylarylamine - Aralkylamine - Aminopyrimidine - Alkyl aryl ether - Benzenoid - Fatty acyl - Fatty amide - Monocyclic benzene moiety - Monosaccharide - Pyrimidine - N-substituted imidazole - Imidolactam - Tetrahydrofuran - Imidazole - Heteroaromatic compound - Azole - Secondary alcohol - Carboxamide group - Secondary carboxylic acid amide - Amino acid or derivatives - Carboxylic acid derivative - Ether - Azacycle - Organoheterocyclic compound - Oxacycle - Hydrochloride - Organic oxide - Carbonyl group - Hydrocarbon derivative - Primary aliphatic amine - Alcohol - Organopnictogen compound - Organonitrogen compound - Organooxygen compound - Amine - Primary alcohol - Primary amine - Organic nitrogen compound - Organic oxygen compound - Aromatic heteropolycyclic compound
Description	This compound belongs to the class of organic compounds known as purine 3'-deoxyribonucleosides. These are compounds consisting of a purine linked to a ribose which lacks a hydroxyl group at position 3.
External Descriptors	Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

Associated Targets(Human)

TDP1 Tchem Tyrosyl-DNA phosphodiesterase 1 (345557 Activities)

Discover Target: TDP1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

TRPM7 Tchem Transient receptor potential cation channel subfamily M member 7 (19 Activities)

Discover Target: TRPM7

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

SLCO1B1 Tchem Solute carrier organic anion transporter family member 1B1 (2672 Activities)

Discover Target: SLCO1B1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Names and Identifiers

Pubchem Sid	488189807
Pubchem Sid Url	https://pubchem.ncbi.nlm.nih.gov/substance/488189807
IUPAC Name	(2S)-2-amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride
INCHI	InChI=1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1
InChIKey	MKSVFGKWZLUTTO-FZFAUISWSA-N
Smiles	COC1=CC=C(C[C@H](N)C(=O)NC2[C@@H](CO)O[C@H]([C@H]2O)N2C=NC3=C2N=CN=C3N(C)C)C=C1
Isomeric SMILES	CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl
WGK Germany	3
RTECS	AU7355000
Alternate CAS	53-79-2
Molecular Weight	544.44
Beilstein	3853613
Reaxy-Rn	39769009
Reaxys-RN_link_address	https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=39769009&ln=

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

11 results found

Lot Number	Certificate Type	Date	Item
E2429495	Certificate of Analysis	May 14, 2024	P432999
E2429496	Certificate of Analysis	May 14, 2024	P432999
E2429522	Certificate of Analysis	May 14, 2024	P432999
K2428187	Certificate of Analysis	May 14, 2024	P432999
G2306302	Certificate of Analysis	Jun 20, 2023	P432999
G2306309	Certificate of Analysis	Jun 20, 2023	P432999
G2306310	Certificate of Analysis	Jun 20, 2023	P432999
G2306306	Certificate of Analysis	Jun 20, 2023	P432999
G2306303	Certificate of Analysis	Jun 20, 2023	P432999
G2306304	Certificate of Analysis	Jun 20, 2023	P432999
B2423043	Certificate of Analysis	Jun 20, 2023	P432999

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Chemical and Physical Properties

Solubility	H2O: soluble 50mg/mL, clear, colorless to faintly yellow
Sensitivity	Heat, moisture and light sensitive
Melt Point(°C)	174°C(lit.)
Molecular Weight	544.400 g/mol
XLogP3
Hydrogen Bond Donor Count	6
Hydrogen Bond Acceptor Count	10
Rotatable Bond Count	8
Exact Mass	543.176 Da
Monoisotopic Mass	543.176 Da
Topological Polar Surface Area	161.000 Å²
Heavy Atom Count	36
Formal Charge	0
Complexity	680.000
Isotope Atom Count	0
Defined Atom Stereocenter Count	5
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
The total count of all stereochemical bonds	0
Covalently-Bonded Unit Count	3

Documents & Articles

Antibiotics block bacterial protein synthesis

Categories: Technical articles

Tags:

Antibiotics Tetra Aminoglycosides Macrolides Peptidyl transferase inhibitors Antibiotics for cell culture

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Jing Zhou, Tongyu Wu, Chun Li, Zhuozhou Hu, Liang Han, Xiangxiang Li, Jingjing Liu, Wenyang Zhao, Jia Kang, Xinping Chen. (2023) Alfuzosin ameliorates diabetes by boosting PGK1 activity in diabetic mice. LIFE SCIENCES, 317 (121491).
2. Song Runjie, Ma Shuoqian, Xu Jiajia, Ren Xin, Guo Peilan, Liu Huijiao, Li Peng, Yin Fan, Liu Mei, Wang Qiang, Yu Lei, Liu Jiali, Duan Binwei, Rahman Nafis A., Wołczyński Sławomir, Li Guangming, Li Xiangdong. (2023) A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR. Molecular Cancer, 22 (1): (1-20).
3. Zhuozhou Hu, Jing Zhou, Liang Han, Xiangxiang Li, Chun Li, Tongyu Wu, Jingjing Liu, Wenyang Zhao, Jia Kang, Xinping Chen. (2022) Acyclovir alleviates insulin resistance via activating PKM1 in diabetic mice. LIFE SCIENCES, 304 (120725).
4. Qiong Yang, Maochen Xing, Ke Wang, Qiang Wei, Jiarui Zhao, Yuan Wang, Kai Ji, Shuliang Song. (2022) Application of Fucoidan in Caco-2 Model Establishment. Pharmaceuticals, 15 (4): (418).
5. Bo Zhang, Ting Jiang, Li Ling, Zhonglian Cao, Jingjing Zhao, Yanyan Tuo, Xiaojian She, Shun Shen, Xinguo Jiang, Yu Hu, Zhiqing Pang. (2016) Enhanced Antitumor Activity of EGFP-EGF1-Conjugated Nanoparticles by a Multitargeting Strategy. ACS Applied Materials & Interfaces, 8 (14): (8918–8927).
6. Jia Liu, Liuting Zheng, Xinyue Li, Wei Tang, Manyu Guo, Yuxing Wang, Xiaoqi Tan, Jiajia Chang, Huiyue Zhao, Dongsheng Zhu, Yu-qiang Ma, Da Huo. (2025) Emerging of Ultrafine Membraneless Organelles as the Missing Piece of Nanostress: Mechanism of Biogenesis and Implications at Multilevels. ACS Nano,
7. Zikang Xing, Xuewen Li, Zhen Ning Tony He, Xin Fang, Heng Liang, Chunxiang Kuang, Aiying Li, Qing Yang. (2024) IDO1 Inhibitor RY103 Suppresses Trp-GCN2-Mediated Angiogenesis and Counters Immunosuppression in Glioblastoma. Pharmaceutics, 16 (7): (870).
8. Ping Yang, Zheng-Jian Wang, Hai-Tao Lu, Xu-Meng Feng, Jing-Long Ye, Guangchuan Wang, Cheng-Feng Qin, Qing Ye, Zhong-Yu Liu. (2024) Imaging of viral replication in live cells by using split fluorescent protein-tagged reporter flaviviruses. VIROLOGY, (110374).
9. Xinrui Meng, Jingjing Liu, Jia Kang, Menghan Wang, Zhanghui Guan, Dong Tian, Xinping Chen. (2024) Lamivudine protects mice from gastric ulcer by activating PGK1 to suppress ferroptosis. BIOCHEMICAL PHARMACOLOGY, 227 (116440).
10. Jingxin Sun, Zhehao Jin, Yong Jin, Haidan Yuan, Guangyu Jin, Jishan Quan. (2025) MRI-Based Multifunctional Nanoliposomes for Enhanced HCC Therapy and Diagnosis. MOLECULAR PHARMACEUTICS,
11. Wei Li, Qingwen Zeng, Bing Wang, Chao Lv, Haoan He, Xi Yang, Bin Cheng, Xiaoan Tao. (2024) Oxidative stress promotes oral carcinogenesis via Thbs1-mediated M1-like tumor-associated macrophages polarization. Redox Biology, 76 (103335).
12. Yao Yongchang, Chen Ke, Pan Qian, Gao Hui, Su Weixian, Zheng Shicong, Dong Weiqiang, Qian Dongyang. (2024) Redifferentiation of genetically modified dedifferentiated chondrocytes in a microcavitary hydrogel. BIOTECHNOLOGY LETTERS, (1-13).
13. Xiuli Zhang, Mingjing Wei, Zhigang Zhang, Yarong Zeng, Feihong Zou, Sibo Zhang, Zhiping Wang, Fentian Chen, Hualong Xiong, Yufang Li, Lizhi Zhou, Tingting Li, Qingbing Zheng, Hai Yu, Jun Zhang, Ying Gu, Qinjian Zhao, Shaowei Li, Ningshao Xia. (2024) Risedronate-functionalized manganese-hydroxyapatite amorphous particles: A potent adjuvant for subunit vaccines and cancer immunotherapy. JOURNAL OF CONTROLLED RELEASE, 367 (13).

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SKU	Size	Availability	Price
P432999-10mg	10mg	5	$88.90
P432999-25mg	25mg	2	$154.90
P432999-100mg	100mg	1	$490.90

Puromycin dihydrochloride from Streptomyces alboniger - powder, BioReagent, suitable for cell culture, high purity , CAS No.58-58-2