Basic Description

Product Name	Proteinase K from Tritirachium album, CAS No.39450-01-6
Grade	for molecular biology, UltraBio™
Product Description	Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Specifications & Purity	UltraBio™, Suitable for molecular biology, lyophilized powder, ≥30 units/mg protein
Biochemical and Physiological Mechanisms	Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad ran

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Organoheterocyclic compounds
  - Class Lactams
    - Subclass Beta lactams

Kingdom	Organic compounds
Superclass	Organoheterocyclic compounds
Class	Lactams
Subclass	Beta lactams
Intermediate Tree Nodes	Not available
Direct Parent	Carbapenems
Alternative Parents	Benzyloxycarbonyls Alpha amino acids and derivatives Nitrobenzenes Pyrroline carboxylic acids Phenoxy compounds Phenol ethers Nitroaromatic compounds Monoalkyl phosphates Azepines Alkyl aryl ethers Vinylogous esters Tertiary carboxylic acid amides Enoate esters Secondary alcohols Azetidines Propargyl-type 1,3-dipolar organic compounds Organic oxoazanium compounds Monocarboxylic acids and derivatives Azacyclic compounds Organopnictogen compounds Organonitrogen compounds Organic oxides Hydrocarbon derivatives Carbonyl compounds
Molecular Framework	Aromatic heteropolycyclic compounds
Substituents	Carbapenem - Benzyloxycarbonyl - Alpha-amino acid or derivatives - Nitrobenzene - Phenoxy compound - Nitroaromatic compound - Pyrroline carboxylic acid or derivatives - Pyrroline carboxylic acid - Phenol ether - Monoalkyl phosphate - Azepine - Alkyl aryl ether - Benzenoid - Alkyl phosphate - Phosphoric acid ester - Organic phosphoric acid derivative - Monocyclic benzene moiety - Vinylogous ester - Alpha,beta-unsaturated carboxylic ester - Enoate ester - Tertiary carboxylic acid amide - Pyrroline - Organic nitro compound - Secondary alcohol - C-nitro compound - Carboxylic acid ester - Carboxamide group - Azetidine - Azacycle - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Allyl-type 1,3-dipolar organic compound - Organic oxoazanium - Monocarboxylic acid or derivatives - Ether - Carboxylic acid derivative - Organic nitrogen compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Organooxygen compound - Organonitrogen compound - Carbonyl group - Alcohol - Aromatic heteropolycyclic compound
Description	This compound belongs to the class of organic compounds known as carbapenems. These are beta-lactam derivatives in which the beta-lactam ring shares the nitrogen atom with a pyrrole-2-carboxylic acid.
External Descriptors	Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

Storage and Shipping

Concentration	lyophilized powder, ≥30 units/mg protein
Storage Temp	Store at -20°C
Shipped In	Ice chest + Ice pads
CAS	39450-01-6
Enzyme Commission Number	3.4.21.64(BRENDA,IUBMB)

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Ziyu Gan, Jun Wang. (2025) A MOF-stabilized enzyme-based colorimetric biosensor integrated with pH test strips for portable detection of D-limonene in agricultural applications. CHEMICAL ENGINEERING JOURNAL, 510 (161719).
2. Wei Bao, Yunbao Gao, Jianing Zhang, Jing Jin, Na Zhang, Baijun Liu, Mingyao Zhang, Zhaohui Su, Hongwen Liang, Xiangling Ji, Wei Jiang. (2025) Biodegradable poly (lactic acid) blends toughened with in situ formed core-shell structure by reactive processing. POLYMER, (128330).
3. Xue Yunfan, Li Xiaoyan, Fan Zhuizhui, Ji Yating, Wang Jie, Chen Qinhu, Cai Zaisheng. (2025) Eco-friendly Anti-felting Finishing and Low-Temperature Dyeing of Wool Through Plant Protease. FIBERS AND POLYMERS, (1-12).
4. Weike Hu. (2024) EP300-mediated H3 acetylation elevates MTHFD2 expression to reduce mitochondrial dysfunction in lipopolysaccharide-induced tubular epithelial cells. RENAL FAILURE,
5. Peng Cui, Jiamin Wang, Lu Mao, Yuan Gao, Shuang Li, Yan Liu, Haimei Mao, Munan Qiu, Wenqi Zou, Xia Gao. (2025) Investigation of biodegradable microparticle release and environmental accumulation in Poly(butylene succinate) degradation under UV and non-UV aging conditions. POLYMER DEGRADATION AND STABILITY, 237 (111323).
6. Yulong Wu, Jin Zhang, Ruikai Zhu, Hong Zhang, Dapeng Li, Huanhuan Li, Honggang Tang, Lihong Chen, Xinyan Peng, Xianrong Xu, Ke Zhao. (2024) Mechanistic Study of Novel Dipeptidyl Peptidase IV Inhibitory Peptides from Goat’s Milk Based on Peptidomics and In Silico Analysis. Foods, 13 (8): (1194).
7. Yu Zhengtao, Xia Ying, Li Jiameng, Jiang Junwen, Li You, Li Youjun, Wang Liu. (2024) METTL3 mediates m6A modification of lncRNA CRNDE to promote ATG10 expression and improve brain ischemia/reperfusion injury through YTHDC1. Biology Direct, 19 (1): (1-17).
8. Wenlong Pang, Bin Li, Yufeng Wu, Yu Zhang, Jun Yang, Zhongxun Tian, Shaonan Tian, Jianjun Li. (2024) One-Step Synthesis of PtPb Alloy Nanoparticles Via Wet Chemical Method for the Upgraded Recycling of PLA Plastic. ACS Sustainable Chemistry & Engineering, 13 (1): (165-173).
9. Shuifang Mao, Yanming Ren, Yujun Zeng, Xingqian Ye, Jinhu Tian. (2025) pH-Responsive EGCG-Metal self-assembly as the carrier for rapid and prolonged insulin delivery. CHEMICAL ENGINEERING JOURNAL, 504 (159048).
10. Ruopeng Yin, Tao Wang, Jingzu Sun, Huanqin Dai, Yuting Zhang, Ningning Liu, Hongwei Liu. (2024) Postbiotics From Lactobacillus Johnsonii Activates Gut Innate Immunity to Mitigate Alcohol-Associated Liver Disease. Advanced Science, (2405781).
11. Shitong Wang, Kai Huang, Wenjing Shi, Jiajia Mo, Honghui Shi, Chuntai Liu, Baobao Chang. (2024) Promoting the formation of stereocomplex crystal in PLLA/PDLA by crosslinking restricted phase separation and solid-state stretching induced orientation. POLYMER, 312 (127670).
12. Xiaofei Wang, Jili Han, Shuxian Zhang, Keshuai Liu, Xiaoxuan Fan, Changcai Bai, Guoning Chen. (2024) Self-polymerization silica nanoparticles based molecularly imprinted polymers for selective recognition of protein. JOURNAL OF CHROMATOGRAPHY A, 1732 (465260).
13. Kanzhen Liu, Cuiping Pang, Qinghua Li, Jianghua Li, Guocheng Du, Guoqiang Zhang. (2025) Step Enzymatic Hydrolysis and In Silico Screening-Assisted Preparation of Bioactive Peptides from Abalone. Foods, 14 (7): (1209).

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SKU	Size	Availability	Price
P755752-5mg	5mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$53.90
P755752-10mg	10mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$65.90
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P755752-5×1g	5×1g	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$5,067.90

Proteinase K from Tritirachium album, CAS No.39450-01-6