Determine the necessary mass, volume, or concentration for preparing a solution.
This is a demo store. No orders will be fulfilled.
Basic Description
| Storage Temp | Store at -20°C | |||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Shipped In |
Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available. |
|||||||||||||||||||||||||||||||||||||||||
| Product Description |
Product introduction: SDS denaturing electrophoresis loading buffer, 4 × （Protein SDS PAGE Loading Buffered Solution, 4 ×） It is suitable for protein loading during SDS-PAGE (SDS denatured polyacrylamide gel electrophoresis). Its main ingredients are SDS, DTT, bromophenol blue, buffer salt solution, etc. SDS can combine with protein to make the protein SDS complex carry a large number of negative charges. At this time, the charge of the protein itself is completely covered by SDS, eliminating the difference in the charge of various proteins; SDS can also break the intramolecular and intermolecular hydrogen bonds and destroy the secondary and tertiary structures of protein molecules. DTT can break the disulfide bond between cysteine residues, destroy the protein structure, and eliminate the differences between protein structures. The electrophoretic migration of proteins (subunits) with no charge and structural differences is only related to their molecular weight. Bromophenol blue is used as an indicator for electrophoresis, which can roughly indicate the end time of electrophoresis. usage method: 1. Dilute the protein sample and the sample buffer solution at a ratio of 3:1 for use. If the concentration of protein sample is too high, it can be diluted with Grade III water or Grade I water. 2. After mixing, heat it in 100 ℃ water bath (or metal bath) for 5 minutes to denature the protein. 3. After cooling to room temperature, centrifugate at 10000-14000 rpm for 2-5 minutes, and then directly load samples for electrophoresis. 4. After protein denaturation, if the protein is stored at room temperature for a long time or stored at low temperature, protein renaturation will occur, and it needs to be reheated for denaturation before electrophoresis. Scope of application: applicable to general biological and medical research Product specifications and parameters:
|
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Solution Calculators
Reviews
Customer Reviews
Remind me later