Prestained Color Protein Marker | Prestained Color Protein Ladder | Broad Multi Color Pre-Stained Protein Standard | Star Ribbon Pre-stained Protein Marker | Tricolor Pre-Stained Protein Marker | Protein Ladder
Grade
10-250kDa
Product Description
Color pre dyed protein molecular weight standard: 1. This product contains 11 highly purified and pre stained recombinant proteins ranging from 10kDa to 250kDa. 2. Suitable as a protein molecular weight standard for SDS-PAGE and Western blotting. 3. This color pre stained protein molecular weight standard has been prepared in 1 × SDS-PAGE buffer and should be used directly without boiling, diluting, or adding reducing agents. 4. According to the size of the sample well, this color pre stained protein molecular weight standard usually requires 5-10 microliters of sample to be loaded each time (5μl for 5 × 1.5mm gel wells is sufficient), and very clear protein bands can be observed during electrophoresis, electrophoresis, and membrane transfer.
The pre stained protein molecular weight standard, calibrated with non pre stained protein molecular weight standards, has the following outstanding advantages: 1. The blue and green bands showed little change in apparent molecular weight on SDS-PAGE gel in Tris Glycine, Bis Tris, and Tris Acetate buffer systems. 2. The orange (red) color stripes are consistent with the changes in imported products.
Usage method: 1. After thawing at room temperature, completely dissolve and gently mix well, do not boil. 2. Take 5µl of this product and test sample for polyacrylamide gel electrophoresis at the same time; It is recommended that laboratories with conditions determine the appropriate sample size through pre experiments based on their own experimental conditions and habits when using this product for the first time. This can save costs and obtain better experimental images. 3. Unused color pre dyed protein molecular weight standards should be stored under storage conditions and can be left at 4°C for 2 months.
Matters needing attention: 1. Pay attention to the electrophoresis time, as low molecular weight proteins may swim at the front edge of the dye. 2. During Western blotting of high molecular weight proteins, it is necessary to extend the transfer time or increase the transfer voltage. Additionally, it is recommended not to add SDS to the transfer solution. If it is necessary for the experiment, the concentration of SDS should not exceed 0.02% -0.04%. 3. Pre stained proteins have different apparent molecular weights in different buffer systems. If non pre stained proteins are calibrated in advance in this buffer system, the protein molecular weight can be roughly determined.
Store at 4℃ short term (2 months). Store at -20℃ long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Prestained Color Protein MW Marker (10-250kDa) (rp192041) - SDS-PAGE band profile in Tris-Glycine lmages are from a Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane. Prestained Color Protein MW Marker (10-250kDa) (rp192041) are mixture of 11 recombinant proteins, 9 blue-stained bands, a green-stained band (25kDa) and an orange-stained band (72kDa).
Prestained Color Protein MW Marker (10-250kDa) (rp192041) - SDS-PAGE band profile in Bis-Tris lmages are from a Bis-Tris gel (SDS-PAGE) and subsequent transfer to membrane. Prestained Color Protein MW Marker (10-250kDa) (rp192041) are mixture of 11 recombinant proteins, 9 blue-stained bands, a green-stained band (25kDa) and an orange-stained band (65kDa).
Prestained Color Protein MW Marker (10-250kDa) (rp192041) - Migration patterns in different electrophoretic conditions The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein using the Prestained Color Protein MW Marker (10-250kDa) (rp192041) in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.
