2 Citations
Determine the necessary mass, volume, or concentration for preparing a solution.
This is a demo store. No orders will be fulfilled.
Basic Description
| Storage Temp | Room temperature | |||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Shipped In | Normal | |||||||||||||||||||||||||||||
| Product Description |
Product introduction: Phosphate buffer solution (PBS) is a kind of buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulas, potassium chloride and potassium phosphate. The weight molar osmotic pressure concentration and ion concentration of the solution are the same as those of human body (isotonic), and they are non-toxic to most cells. Product application: Phosphoric acid buffer salt has been used to produce platinum nanoparticles (NP Pt) hydrolytic colloid to wash blood samples before SEM and to prepare SEM (scanning electron microscope) stub to prepare fluorescent labeled wheat germ agglutinin (WGA) solution. During ELISA (enzyme-linked immunosorbent assay), dilute and capture antibodies. During centrifugation, suspend cells to precipitate immunoassay procedures usage method: 1. Dissolve the product in Grade III water or Grade I water and dilute it to 10ml to obtain 10ml of concentrated 10X phosphate buffer salt solution. 2. If it is necessary to prepare 1X concentration phosphate buffer salt solution, the product can be dissolved in tertiary water or primary water to a constant volume of 100ml, or 10X concentrated solution can be diluted with tertiary water or primary water in a ratio of 1:9. 3. The phosphate buffer salt solution obtained after dissolution passes 0.22 μ M After filtration and sterilization, it can be used for cell biology experiment. The endotoxin in the preparation water should be less than 0.01EU/ml and be packed in a sterile container without endotoxin. 4. Steam sterilization will cause solution volume loss and change of solution concentration, pH, osmotic pressure and other parameters. Do not use steam sterilization to sterilize the solution. 5. After dissolution, the pH of the product can be stabilized within the marked range at 25 ℃ - 37 ℃, without further adjustment with HCl. If you need phosphate buffer salt solution of other pH, you can purchase the corresponding product or customize it. Product specifications and parameters:
|
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Citations of This Product
| 1. Zixiong Song, Ying Jing, Xiaoxiao Wei, Haizhen Li, Jianhua Xie, Mingyue Shen. (2025) Mechanistic insights into advanced glycation end products production in glucose-lysine model system involved with ascorbic acid. FOOD CHEMISTRY, 474 (143178). |
| 2. Chao-fan He, Tian-hong Qiao, Xu-chao Ren, Mingjun Xie, Qing Gao, Chao-qi Xie, Peng Wang, Yuan Sun, Huayong Yang, Yong He. (2025) Printability in Multi-material Projection-Based 3-Dimensional Bioprinting. Research, 8 (0613). |
Solution Calculators
Reviews
Customer Reviews
Remind me later