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Phaseolus vulgaris lectin P (PHA-P), native isomer composition

In stock
Item Number
P419332
Grouped product items
SKU Size
Availability
Price Qty
P419332-5mg
5mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$534.90
P419332-10mg
10mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$900.90

Basic Description

Storage Temp Store at -20°C
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Product Description

Features 

Ultrapure quality

Agglutinates leucocytes and erythrocytes

Mixture of L and E subunits

Sugar specificity: complex oligosaccharides

Lyophilized powder

 

Product description

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P)is isolated from red kidney beans and purified by affinitychromatography. PHA-P is a mixture of isolectins containingL (leucoagglutinin) and E (erythroagglutinin) subunits incompositions L1E3, L2E2, L3E1, E4.

Thus PHA-P is the form of PHA before separation oferythroagglutinin and leucoagglutinin. PHA-P has amolecular weight of 128 kDa and specificity for complexoligosaccharides . PHA-P is not blood group specific and agglutinates human erythrocytes blood group 0 with alectin concentration of ≤4 μg/ml.

PHA-P lectin is supplied as a white to light-pinkessentially salt-free lyophilized powder from 50 mM NH4HCO3,which evaporates during the lyophilization procedure. Thepurity of the lectin is determined by SDS-PAGE generatingone single band.


Applications

Lateral flow erythrocyte separation

Typical subunit composition

Total L subunit composition: 60-65%

Total E subunit composition: 35-40%

L4 25-35%

L3E1 20-30%

L2E2 10-20%

L1E3 17-27%

E4 1-11%


Directions for use

The lectin may be reconstituted in a suitable buffer for yourapplication.


Shipping and storage

The product is shipped at -20°C, however for over-the-daytransport it may be shipped at ambient temperature. Thelyophilized powder is stable for more than five years fromproduction date when stored below -20°C. After reconstitutionwith PBS, pH 7.4, the solution may be stored frozen in workingaliquots for up to 12 months.


Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

5 results found

Lot Number Certificate Type Date Item
E2315281 Certificate of Analysis Feb 14, 2023 P419332
E2312406 Certificate of Analysis Feb 14, 2023 P419332
D23062072 Certificate of Analysis Feb 14, 2023 P419332
D23062068 Certificate of Analysis Feb 14, 2023 P419332
D23062070 Certificate of Analysis Feb 14, 2023 P419332

Solution Calculators

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