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SKU | Size | Availability |
Price | Qty |
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Ab175852-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$44.90
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Ab175852-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$133.90
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Ab175852-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$213.90
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Ab175852-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,279.90
|
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mAb (C04/3D7); Mouse anti Human PCNA Antibody (Biotin); WB; IF/ICC
Product Name | PCNA Mouse mAb (Biotin) - Primary antibody, specific to PCNA |
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Synonyms | ATLD2 antibody | cb16 antibody | Cyclin antibody | DNA polymerase delta auxiliary protein antibody | etID36690.10 antibody | fa28e03 antibody | fb36g03 antibody | HGCN8729 antibody | MGC8367 antibody | Mutagen-sensitive 209 protein antibody | OTTHUMP00000 |
Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
Biochemical and Physiological Mechanisms | Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple prote |
Host species | Mouse |
Specificity | PCNA |
Immunogen | Full length recombinant human PCNA |
Positive Control | WB: HeLa, K562, Hep G2 whole cell lysates IF/ICC: Hela cells |
Conjugation | Biotin |
Grade | ExactAb™, Validated |
Product Description |
Mouse anti Human PCNA Antibody, Monoclonal (C04/3D7), could be used for WB and IF/ICC. |
Light Chain Type | kappa |
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Purification Method | Protein A purified |
Concentration | 0.5 mg/mL |
Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Shipped In | Ice chest + Ice pads |
Stability And Storage | Store at -20°C. Protect from light. Avoid freeze/thaw cycles. |
Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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PCNA Mouse mAb (Biotin) (Ab175852) - ICC/IF
IF analysis of PCNA (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (Biotin) (Ab175852) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (LumiDye™ Yellow-Green 568) (rp220558) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
PCNA Mouse mAb (Biotin) (Ab175852) - Western Blot
All lanes: PCNA Mouse mAb (Biotin) (Ab175852) at 1/8000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Streptavidin (HRP) (np156148) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 120.0 s