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PCNA Mouse mAb (Biotin) - Primary antibody, specific to PCNA

    Application:
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: C04/3D7
  • Conjugation: Biotin
In stock
Item Number
Ab175852
Grouped product items
SKU Size
Availability
Price Qty
Ab175852-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$44.90
Ab175852-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$133.90
Ab175852-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$213.90
Ab175852-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,279.90

mAb (C04/3D7); Mouse anti Human PCNA Antibody (Biotin); WB; IF/ICC

Basic Description

Product Name PCNA Mouse mAb (Biotin) - Primary antibody, specific to PCNA
Synonyms ATLD2 antibody | cb16 antibody | Cyclin antibody | DNA polymerase delta auxiliary protein antibody | etID36690.10 antibody | fa28e03 antibody | fb36g03 antibody | HGCN8729 antibody | MGC8367 antibody | Mutagen-sensitive 209 protein antibody | OTTHUMP00000
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple prote
Host species Mouse
Specificity PCNA
Immunogen Full length recombinant human PCNA
Positive Control WB: HeLa, K562, Hep G2 whole cell lysates IF/ICC: Hela cells
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Mouse anti Human PCNA Antibody, Monoclonal (C04/3D7), could be used for WB and IF/ICC.

Product Properties

Light Chain Type kappa
Purification Method Protein A purified
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Associated Targets(Human)

PCNA Tchem Proliferating cell nuclear antigen (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

PCNA Mouse mAb (Biotin) (Ab175852) - ICC/IF
IF analysis of PCNA (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (Biotin) (Ab175852) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (LumiDye™ Yellow-Green 568) (rp220558) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

PCNA Mouse mAb (Biotin) (Ab175852) - Western Blot
All lanes: PCNA Mouse mAb (Biotin) (Ab175852) at 1/8000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Streptavidin (HRP) (np156148) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 120.0 s

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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