Skip to the beginning of the images gallery

PCNA Mouse mAb (AF488) - Primary antibody, specific to PCNA

COA

Grade & Purity:

Validated

ExactAb™

0.5 mg/mL

Application:

Flow Cytometry
IF/ICC

Host species: Mouse
Reactive species: Human,Mouse,Rat
Isotype: Mouse IgG1
Clone Number: C04/3D7
Conjugation: AF488

In stock

Item Number

Ab219142

Grouped product items
SKU	Size	Availability	Price
Ab219142-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$59.90
Ab219142-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$169.90
Ab219142-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$259.90
Ab219142-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,599.90

mAb (C04/3D7); Mouse anti Human PCNA Antibody (AF488); Flow Cytometry; IF/ICC

View related series

Click to view target information: PCNA

Basic Description

Product Name	PCNA Mouse mAb (AF488) - Primary antibody, specific to PCNA
Specifications & Purity	ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms	Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple prote
Host species	Mouse
Specificity	PCNA
Immunogen	Full length recombinant human PCNA
Positive Control	IF/ICC: Hela cells Flow Cytometry: Hela cells
Conjugation	AF488
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	525nm
Ex/Em(nm)	Ex:490nm;Em:525nm
Grade	ExactAb™, Validated
Product Description	Mouse anti Human PCNA Antibody, Monoclonal (C04/3D7), could be used for Flow Cytometry and IF/ICC.

Product Properties

Light Chain Type	kappa
Purification Method	Protein A purified
Concentration	0.5 mg/mL
Storage Temp	Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Associated Targets(Human)

PCNA Tchem Proliferating cell nuclear antigen (0 Activities)

Discover Target: PCNA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

ICC/IF: PCNA Mouse mAb (AF488) (Ab219142)

PCNA Mouse mAb (AF488) (Ab219142) - ICC/IF
IF analysis of PCNA (Green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (AF488) (Ab219142) at a dilution of 1/1000 in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: PCNA Mouse mAb (AF488) (Ab219142)

PCNA Mouse mAb (AF488) (Ab219142) - Flow Cytometry
Flow cytometry analysis of PCNA expression by Hela cells (Red) compared to an isotype control (Blue). Cells were fixed and permeabilized with 100% methanol and then stained with PCNA Mouse mAb (AF488) (Ab219142) (Red) at 1/150 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF488) (Ab190245).

Application

Application	Dilution info
IF/ICC	1:50-1:500
Flow Cytometry	1:50-1:500

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration