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PCNA Mouse mAb (AF405) - Primary antibody, specific to PCNA

    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: C04/3D7
  • Conjugation: AF405
In stock
Item Number
Ab219140
Grouped product items
SKU Size
Availability
Price Qty
Ab219140-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab219140-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$169.90
Ab219140-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$259.90
Ab219140-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,599.90

mAb (C04/3D7); Mouse anti Human PCNA Antibody (AF405); Flow Cytometry; IF/ICC

Basic Description

Product Name PCNA Mouse mAb (AF405) - Primary antibody, specific to PCNA
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple prote
Host species Mouse
Specificity PCNA
Immunogen Full length recombinant human PCNA
Positive Control IF/ICC: Hela cells Flow Cytometry: Hela cells
Conjugation AF405
Excitation(nm) Violet Laser(405nm)
Emission(nm) 422nm
Ex/Em(nm) Ex:401nm;Em:422nm
Grade ExactAb™, Validated
Product Description

Mouse anti Human PCNA Antibody, Monoclonal (C04/3D7), could be used for Flow Cytometry and IF/ICC.

Product Properties

Light Chain Type kappa
Concentration 0.5 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Associated Targets(Human)

PCNA Tchem Proliferating cell nuclear antigen (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

PCNA Mouse mAb (AF405) (Ab219140) - ICC/IF
IF analysis of PCNA (Blue) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (AF405) (Ab219140) at a dilution of 1/1000 in blocking buffer overnight at 4°C. Cells were counterstained with PI (Red). Images were taken on the confocal laser scanning microscope.

PCNA Mouse mAb (AF405) (Ab219140) - Flow Cytometry
Flow cytometry analysis of PCNA expression by Hela cells (Red) compared to an isotype control (Blue). Cells were fixed and permeabilized with 100% methanol and then stained with PCNA Mouse mAb (AF405) (Ab219140) (Red) at 1/150 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF405) (Ab190076).

Application

Application Dilution info
IF/ICC

1:50-1:500

Flow Cytometry

1:50-1:500

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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